Light‐Harvesting Artificial Cells for the Generation of ATP and NADPH†. Issue 1 (23rd November 2022)

• Record Type: Journal Article
• Authors: Su, Ziwei; Zhang, Xiangxiang; Wang, Weichen; Dong, Mingdong; Han, Xiaojun
• Is Part Of: Chinese journal of chemistry. Volume 41:Issue 1(2023)
• Page Start: 57
• Page End: 63
• Publication Date: 2022-11-23
• Subjects: Artificial cells -- Thylakoids -- Metabolism -- Enzymes -- Vesicles -- Light harvesting -- ATP -- NADPH -- GUVs; Chemistry -- Periodicals; 540.5
• DOI: 10.1002/cjoc.202200482
• Languages: English
• ISSNs: 1001-604X

Abstract

Comprehensive Summary: The bottom‐up construction of self‐powered artificial cells is significant to understand the energy supply and metabolism of nature cells. Here, we demonstrate an efficient manner to build thylakoid‐containing artificial cells, which continuously convert light energy into chemical energy to supply adenosine 5'‐triphosphate (ATP) under light illumination. The production of ATP supplies energy to promote the biological enzyme cascade reactions, where glucose is transformed into glucose 6‐phosphate (G6P) under the catalysis of hexokinase (HK). G6P was further converted to gluconolactone 6‐phosphate (PG) in the presence of 6‐phosphate dehydrogenase (G6PDH), meanwhile NADP + was converted to nicotinamide adenine dinucleotide phosphate (NADPH). The self‐powered artificial cells were demonstrated to generate ATP and NADPH successively, which provided a way for building more complicated artificial cells. Abstract : Self‐powered artificial cells were developed by encapsulating thylakoids, which continuously convert light energy into chemical energy to produce ATP molecules under illumination. The production of ATP supplies energy for the biological enzyme cascade reactions to promote the generation of NADPH. The self‐powered artificial cells were demonstrated to generate ATP and NADPH inside GUVs.