Potential Wound Healing of PLGA Nanoparticles Containing a Novel L-Carnitine–GHK Peptide Conjugate. (5th December 2022)

• Record Type: Journal Article
• Authors: Ramezanpour, Sorour; Tavatoni, Pedram; Akrami, Mohammad; Navaei-Nigjeh, Mona; Shiri, Pezhman
• Other Names: Premkumar Thathan Academic Editor.
• Is Part Of: Journal of nanomaterials. Volume 2022(2022)
• Publication Date: 2022-12-05
• Subjects: Nanostructured materials -- Periodicals; Nanotechnology -- Periodicals; Nanomatériaux; Nanostructured materials; Nanotechnology; Nanostructures; Nanotechnology; Periodicals; Fulltext; Internet Resources; Periodicals; 620.115
• DOI: 10.1155/2022/6165759
• Languages: English
• ISSNs: 1687-4110

Abstract

Abstract : The development of a product that has simultaneous wound healing, anti-inflammatory, and antimicrobial properties is desirable for wound healing medicine. In this study, glycine–histidine–lysine (GHK) peptide as a skin repair accelerator was coupled to L -carnitine with antibacterial and anti-inflammatory properties to investigate its new wound healing properties in a nanoparticle (NP) platform. The conjugate was synthesized by solid-phase synthesis method with Fmoc chemistry, purified by preparative HPLC, and was identified with ESI-Mass technique. The conjugate was then loaded into PLGA NPs, which was prepared using solvent evaporation technique. Zetasizer results showed a mean size and zeta potential of 193.15 nm and −30.2 ± 3.8 mV for the conjugate-loaded PLGA NPs. Scanning electron microscopy (SEM) exhibited spherical-shaped morphology for the NPs with uniform size distribution (PDI = 0.265). Conjugate release from the NPs was about 10% at initial time, which increased to more than about 70% at 200 hr, exhibiting a sustained release trend. Additionally, the treatment of fibroblasts with the nanoconjugate indicated its biocompatibility. Compared to GHK, L -carnitine and free conjugates, the most satisfactory wound healing activity was observed for conjugate-loaded NPs upon wound closure in a rat model of skin injury repair. Furthermore, more epithelialization and neovascularization were observed for L -carnitine–GHK nanoconjugate. Taken together, the L -carnitine–GHK conjugate-loaded PLGA NP will be a promising candidate for wound healing management.