A 2, 7-dichlorofluorescein derivative to monitor microcalcifications. Issue 11 (19th October 2022)

• Record Type: Journal Article
• Authors: Tholen, Patrik; Brown, Connor N.; Keil, Claudia; Bayir, Ali; Zeng, Hui-Hui; Haase, Hajo; Thompson, Richard B.; Lengyel, Imre; Yücesan, Gündoğ
• Is Part Of: Molecular Systems Design and Engineering. Volume 7:Issue 11(2022)
• Page Start: 1415
• Page End: 1421
• Publication Date: 2022-10-19
• Subjects: Chemistry -- Molecular aspects -- Periodicals; Chemical engineering -- Molecular aspects -- Periodicals; Nanotechnology -- Periodicals; 620.5
• DOI: 10.1039/d2me00185c
• Languages: English
• ISSNs: 2058-9689

Abstract

Abstract : Herein, we report the crystal structure of 2, 7-dichlorofluorescein methyl ester (DCF-ME) and its fluorescence response to hydroxyapatite binding. Abstract : Herein, we report the crystal structure of 2, 7-dichlorofluorescein methyl ester (DCF-ME) and its fluorescence response to hydroxyapatite binding. The reported fluorophore is very selective for staining the bone matrix and provides turn-on fluorescence upon hydroxyapatite binding. The reported fluorophore can readily pass the cell membrane of the C2C12 cell line, and it is non-toxic for the cell line. The reported fluorophore DCF-ME may find applications in monitoring bone remodeling and microcalcification as an early diagnosis tool for breast cancer and age-related macular degeneration.