Determination of L-tryptophan and L-kynurenine in Human Serum by using LC-MS after Derivatization with (R)-DBD-PyNCS. (January 2013)

• Record Type: Journal Article
• Authors: Ohashi, Hayato; Iizuka, Hideaki; Yoshihara, Shunsuke; Otani, Hayato; Kume, Misato; Sadamoto, Kiyomi; Ichiba, Hideaki; Fukushima, Takeshi
• Is Part Of: International journal of tryptophan research. Volume 6(2013)Supplement 1
• Publication Date: 2013-01
• Subjects: (R)-(–)-4-(N, N-dimethylaminosulfonyl)-7-(3-isothiocyanatopyrrolidin-1-yl)-2, 1, 3-benzoxadiazole (DBD-PyNCS) -- high-performance liquid chromatography -- human serum -- L-kynurenine -- L-tryptophan -- mass spectrometry; Biochemistry -- Periodicals; Tryptophan -- Periodicals; Tryptophan; Biochemical Phenomena; Biochemistry; Tryptophan; Electronic journals; Periodicals; Periodicals; 572.65
• DOI: 10.4137/IJTR.S11459
• Languages: English
• ISSNs: 1178-6469

Abstract

Concentrations of L-tryptophan (L-Trp) and its metabolite, L-kynurenine (L-KYN), in sera of 19 normal subjects (age: 23.6 ± 3.5 y, male: 8, female: 11) were determined by high-performance liquid chromatography with mass-spectrometric detection, following their derivatization with ( R)-(–)-4-(N, N -dimethylaminosulfonyl)-7-(3-isothiocyanatopyrrolidin-1-yl)-2, 1, 3-benzoxadiazole (DBD-PyNCS). A significant positive correlation between L-Trp and L-KYN concentrations was observed (r = 0.532, P < 0.05). Serum L-Trp concentration in male subjects (95.65 ± 4.27 μM) was significantly higher than that in female subjects (79.20 ± 3.34 μM; P < 0.05), while no significant differences in L-KYN concentration or the L-KYN:L-Trp ratio were observed between male and female subjects.