Basophil activation test has high reproducibility and is feasible in the clinical setting. Issue 11 (1st November 2022)

• Record Type: Journal Article
• Authors: Jaumdally, Hannah; Kwok, Matthew; Jama, Zainab; Hesse‐Lamptey, Rochelle; McKendry, Richard; Galvez, Oliver; Daniel, Yvonne; Santos, Alexandra F.
• Is Part Of: Pediatric allergy and immunology. Volume 33:Issue 11(2022)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2022-11-01
• Subjects: anaphylaxis -- basophil activation test -- CD203c -- CD63 -- diagnosis -- food allergy -- oral food challenge -- peanut allergy; Allergy in children -- Periodicals; Immunologic diseases in children -- Periodicals; 617
• DOI: 10.1111/pai.13870
• Languages: English
• ISSNs: 0905-6157

Abstract

Abstract: Background: The basophil activation test (BAT) has high accuracy to diagnose peanut allergy and can reduce the need for oral food challenges (OFC); however, so far it has not been incorporated in clinical practice. Methods: We assessed the reproducibility of BAT within the same laboratory and between two different laboratories and the feasibility of using BAT in the clinical setting. Results: One hundred and two children being assessed for peanut allergy were tested on BAT (72 allergic, 30 sensitized tolerant). There was little internal variation (coefficient of variation <15%) in the BAT and a very strong correlation ( R s > .95) between BAT performed across laboratories. The 2 BAT methods were strongly correlated but not interchangeable. In the cases of discrepancy, our in house BAT method was 100% accurate. BAT was feasible and well‐accepted by clinicians: no patient with positive BAT was referred for OFC, leading to reduction in the number of OFC required. Twenty one percent of patients who underwent OFC reacted to peanut. A negative BAT also encouraged the performance of OFC in sensitized children who would otherwise be considered allergic, 50% of whom did not react and incorporated peanut in the diet. Conclusions: The BAT is a robust test that can reliably be transferred between laboratories; however, different BAT methods are not interchangeable. BAT was well integrated in the clinical decision‐making process in a specialized center. Abstract :