Deacetyl epoxyazadiradione protects aminoglycoside antibiotic‐induced renal cell apoptosis, in vitro. (29th September 2022)

• Record Type: Journal Article
• Authors: Murugan, Raghul; Rajesh, Ravi; Velayutham, Manikandan; Juliet, Annie; Gopinath, Pushparathinam; Arockiaraj, Jesu
• Is Part Of: Cell biology international. Volume 47:Number 1(2023)
• Page Start: 123
• Page End: 134
• Publication Date: 2022-09-29
• Subjects: apoptosis -- deacetyl epoxyazadiradione -- free radicals -- MDCK cells -- nephroprotection; Cytology -- Periodicals; Cells -- Periodicals; 571.605
• DOI: 10.1002/cbin.11915
• Languages: English
• ISSNs: 1065-6995

Abstract

Abstract: Aminoglycoside antibiotics such as gentamicin are used frequently to treat bacterial infections in humans. Excessive consumption of these antibiotics lead to renal dysfunction. One of the factors contributing to renal dysfunction is oxidative damage, which causes apoptosis. Hence, this study investigates the effect of the antioxidant compound deacetyl epoxyazadiradione (DEA) in reducing cell death induced by gentamicin treatment in kidney cells (Madin–Darby canine kidney cells). The antioxidant experiments showed that reactive oxygen species level is decreased up to 27.06 ± 0.18% in 150 µM of DEA treatment. At this concentration, the activity of antioxidant enzymes such as superoxide dismutase increased from 0.4 ± 0.04 to 1.46 ± 0.05 µmol/min/L and catalase increased from 7.48 ± 0.39 to 17.6 ± 0.74 U/mg. The relative folds of gene expression of mitochondrial enzymes such as GST, GPx and GR restored from 0.596 ± 0.019, 0.521 ± 0.013 and 0.775 ± 0.014 to 0.866 ± 0.013, 0.669 ± 0.015 and 0.8615 ± 0.028, respectively. Consequently, the percentage of cell viability increases upto 91.8 ± 2.01 from 61.93 ± 1.63 with much less fragmentation in genomic DNA. Additionally, molecular docking results showed that DEA could bind to Bax, Bcl‐ 2, Caspase‐ 3 and Caspase‐ 9 proteins. These results indicate that DEA could reduce cell apoptosis by reducing oxidative stress due to antibiotics and interrupting the apoptotic signal pathway in kidney cells.