Protein charge parameters that influence stability and cellular internalization of polyelectrolyte complex micelles. Issue 48 (29th November 2022)

• Record Type: Journal Article
• Authors: Kapelner, Rachel A.; Fisher, Rachel S.; Elbaum-Garfinkle, Shana; Obermeyer, Allie C.
• Is Part Of: Chemical science. Volume 13:Issue 48(2022)
• Page Start: 14346
• Page End: 14356
• Publication Date: 2022-11-29
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/d2sc00192f
• Languages: English
• ISSNs: 2041-6520

Abstract

Abstract : The stability and delivery efficiency of protein-based polyelectrolyte-complex micelles was evaluated for a panel of proteins with varying net charge and charge distribution. Abstract : Proteins are an important class of biologics, but there are several recurring challenges to address when designing protein-based therapeutics. These challenges include: the propensity of proteins to aggregate during formulation, relatively low loading in traditional hydrophobic delivery vehicles, and inefficient cellular uptake. This last criterion is particularly challenging for anionic proteins as they cannot cross the anionic plasma membrane. Here we investigated the complex coacervation of anionic proteins with a block copolymer of opposite charge to form polyelectrolyte complex (PEC) micelles for use as a protein delivery vehicle. Using genetically modified variants of the model protein green fluorescent protein (GFP), we evaluated the role of protein charge and charge localization in the formation and stability of PEC micelles. A neutral-cationic block copolymer, poly(oligoethylene glycol methacrylate- block -quaternized 4-vinylpyridine), POEGMA79 - b -qP4VP175, was prepared via RAFT polymerization for complexation and microphase separation with the panel of engineered anionic GFPs. We found that isotropically supercharged proteins formed micelles at higher ionic strength relative to protein variants with charge localized to a polypeptide tag. We then studied GFP delivery by PEC micelles and found that they effectively delivered the protein cargo to mammalian cells. However, cellular delivery varied as a function of protein charge and charge distribution and we found an inverse relationship between the PEC micelle critical salt concentration and delivery efficiency. This model system has highlighted the potential of polyelectrolyte complexes to deliver anionic proteins intracellularly. Using this model system, we have identified requirements for the formation of PEC micelles that are stable at physiological ionic strength and that smaller protein–polyelectrolyte complexes effectively deliver proteins to Jurkat cells.