Development and evaluation of osteogenesis and antibacterial properties of strontium/silver-functionalized hierarchical micro/nano-titanium implants. (December 2022)

• Record Type: Journal Article
• Authors: Yao, Litao; Wang, Haiyan; Li, Li; Cao, Zheng; Dong, Yilong; Yao, Lili; Lou, Weiwei; Zheng, Shutian; Shi, Yizuo; Shen, Xinkun; Cai, Chunyuan; Sheng, Lieping
• Is Part Of: Materials & design. Volume 224(2022)
• Publication Date: 2022-12
• Subjects: Titanium -- Magnetron sputtering -- Bilayer coating -- Antibacterial -- Osteogenesis; Materials -- Periodicals; Engineering design -- Periodicals; Matériaux -- Périodiques; Conception technique -- Périodiques; Electronic journals; 620.11
• DOI: 10.1016/j.matdes.2022.111425
• Languages: English
• ISSNs: 0264-1275

Abstract

Graphical abstract: Highlights: The release rate of Ag + from inner silver layer can be controlled by the covering of SrTiO3 nanoparticle coating. The SrTiO3 nanoparticle coating did not impede the antibacterial activity of Ag-modified samples. The AH-Ti/Ag/Sr substrate possessed the dual functions of osteogenesis and antibacterial activity. The controlled Ag + release and the suitable Sr 2+ release had a synergistic effect on osteointegration. Abstract: Promoting implant osseointegration while preventing infection is one of the major challenges in the development of dental implants. In this study, nano-needle-like structures were prepared on titanium by hot alkali treatment and then coated with uniform silver (Ag) and strontium titanate (SrTiO3 ) through magnetron sputtering. The results of ion release showed that the SrTiO3 outer coating reduced the release of Ag ions by 30% and 15% respectively on the 4th and 7th days, thus effectively improving the bioactivity of the Ag-modified substrates. In vitro experiments demonstrated that the Ag/SrTiO3 -coated specimens (AH-Ti/Ag/Sr) had an excellent effect on the osteogenic differentiation of MC3T3-E1 cells and exhibited antibacterial function against Staphylococcus aureus and Escherichia coli (percentage of dead/total bacteria: ∼93% and ∼88%). Moreover, the in vivo results further confirmed that the Ag/SrTiO3 bilayer coating significantly promoted osteogenesis around the AH-Ti/Ag/Sr implant (the new bone volume and the number of trabeculae: 1.5 and 1.4 fold of the control group) and enhanced the bonding strength between implant and surrounding bone (about 1.9 fold of the control group). All these findings indicate that AH-Ti/Ag/Sr can substantially improve osteogenesis in complex oral microenvironment, and thus has potential applications in oral implantology.