Molecular identification and lipid mobilization role of adipokinetic hormone receptor in Spodoptera litura (F.). Issue 6 (18th December 2022)

• Record Type: Journal Article
• Authors: Dong, Xiaolin; Chen, Junyuan; Xu, Rubing; Li, Xihong; Wang, Yang; Pan, Xue; Zhang, Cuici; Li, Yanyan; Wang, Fulian; Li, Chuanren
• Is Part Of: Bulletin of entomological research. Volume 112:Issue 6(2022)
• Page Start: 758
• Page End: 765
• Publication Date: 2022-12-18
• Subjects: Adipokinetic hormone receptor -- fat body -- free fatty acid -- triacylglycerol; Entomology -- Periodicals; 595.7
• DOI: 10.1017/S0007485322000141
• Languages: English
• ISSNs: 0007-4853

Abstract

Abstract: Energy homeostasis is essential for organisms to maintain fluctuation in energy accumulation, mobilization. Lipids as the main energy reserve in insects, their metabolism is under the control of many physiological program. This study aimed to determine whether the adipokinetic hormone receptor (AKHR) was involved in the lipid mobilization in the Spodoptera litura . A full-length cDNA encoding AKHR was isolated from S. litura . The Sl AKHR protein has a conserved seven-transmembrane domain which is the character of a putative G protein receptor. Expression profile investigation revealed that SlAKHR mRNA was highly expressed in immatural stage and abundant in fat body in newly emerged female adults. Knockdown of SlAKHR expression was achieved through RNAi by injecting double-stranded RNA (dsRNA) into the 6th instar larvae. The content of triacylgycerol (TAG) in the fat body increased significantly after the SlAKHR gene was knockdown. And decrease of TAG releasing to hemolymph with increase of free fatty acid (FFA) in hemolymph were observed when the SlAKHR gene was knowned-down. In addition, lipid droplets increased in fat body was also found. These results suggested that SlAKHR is critical for insects to regulate lipids metabolism.