Identification and differential expression of human carcinoma-associated antigens in hepatocellular carcinoma tissues. (February 2013)

• Record Type: Journal Article
• Authors: Zhou, Dong-Xun; Zheng, Long-Yi; Qu, Hai-Xia; Yu, Feng-Hai; Zou, Shan-Shan; Hong, Yi; Tan, Ye-Xiong; Wang, Hao; Hu, He-Ping; Wang, Hong-Yang
• Is Part Of: Experimental biology and medicine. Volume 238:Number 2(2013)
• Page Start: 167
• Page End: 175
• Publication Date: 2013-02
• Subjects: human carcinoma-associated antigen -- hepatocellular carcinoma -- monoclonal antibody -- CLIMP63; Physiology -- Periodicals; Biology, Experimental -- Periodicals; Medicine, Experimental -- Periodicals; 610.72
• DOI: 10.1177/1535370212474599
• Languages: English
• ISSNs: 1535-3702

Abstract

This study was designed to identify and verify hepatocellular carcinoma (HCC)-associated human carcinoma antigens (HCAs) that may be useful as tumor markers for HCC. We found that BCE075 and BCD021 anti-HCA antibodies were immunostained in the liver tissue samples and showed specific staining. Their expression was increased in HCC compared with normal liver tissues ( P = 0.008). Immunoprecipitation and mass spectrometry analyses of the proteins precipitated by these two antibodies were identified to be cytoskeleton-associated protein 4 (CLIMP63) and brain-type glycogen phosphorylase (PYGB). This study demonstrated that HCC tissues expressed specific HCA glycoproteins, suggesting that our mouse monoclonal anti-HCA antibodies could be useful for immunohistochemical analysis of HCA expression as potential biomarkers for HCC diagnosis.