CircRNA_45478 promotes ischemic AKI by targeting the miR‐190a‐5p/PHLPP1 axis. Issue 12 (31st October 2022)

• Record Type: Journal Article
• Authors: Liao, Yingjun; Peng, Xiongjun; Li, Xiaozhou; Wu, Dengke; Qiu, Shuangfa; Tang, Xianming; Zhang, Dongshan
• Is Part Of: FASEB journal. Volume 36:Issue 12(2022)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2022-10-31
• Subjects: AKI -- apoptosis -- circular RNA -- miR‐190a‐5p -- PHLPP1; Biology -- Periodicals; Biology, Experimental -- Periodicals; 570
• DOI: 10.1096/fj.202201070R
• Languages: English
• ISSNs: 0892-6638

Abstract

Abstract: A few studies suggested that circular RNAs were involved in the development of ischemic acute kidney injury (AKI). However, the function and regulation mechanism of circRNA_45478 in ischemic AKI remains unknown. In the present study, ischemic injury induced the expressions of circRNA_45478 in mouse proximal tubule‐derived cell lines (BUMPT cells) and kidneys of C57BL/6 mice. Functionally, circRNA_45478 mediated I/R‐induced apoptosis in BUMPT cells. Mechanistically, circRNA_45478 upregulated the expression of Pleckstrin homology (PH) domain leucine‐rich repeat protein phosphatase 1 (PHLPP1) via sponging of microRNA (miR)‐190a‐5p. Finally, inhibition of circRNA_45478 significantly alleviated the progression of ischemic AKI through regulation of the miR‐190a‐5p/PHLPP1 pathway. Taken together, our data showed that circRNA_45478/miR‐190a‐5p/PHLPP1 axis mediated the progression of ischemic AKI.