General‐Purpose Ultrasound Neuromodulation System for Chronic, Closed‐Loop Preclinical Studies in Freely Behaving Rodents. Issue 34 (19th October 2022)

• Record Type: Journal Article
• Authors: Jo, Yehhyun; Lee, Sang‐Mok; Jung, Taesub; Park, Gijae; Lee, Chanhee; Im, Geun Ho; Lee, Seongju; Park, Jin Soo; Oh, Chaerin; Kook, Geon; Kim, Hyunggug; Kim, Seongyeon; Lee, Byung Chul; Suh, Greg S.B.; Kim, Seong‐Gi; Kim, Jeongyeon; Lee, Hyunjoo J.
• Is Part Of: Advanced science. Volume 9:Issue 34(2022)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2022-10-19
• Subjects: closed‐loop systems -- preclinical studies -- rapid eye movement (REM) sleep modulation -- spatial working memory -- therapeutics -- ultrasound stimulation; Science -- Periodicals; 505
• DOI: 10.1002/advs.202202345
• Languages: English
• ISSNs: 2198-3844

Abstract

Abstract: Transcranial focused ultrasound stimulation (tFUS) is an effective noninvasive treatment modality for brain disorders with high clinical potential. However, the therapeutic effects of ultrasound neuromodulation are not widely explored due to limitations in preclinical systems. The current preclinical studies are head‐fixed, anesthesia‐dependent, and acute, limiting clinical translatability. Here, this work reports a general‐purpose ultrasound neuromodulation system for chronic, closed‐loop preclinical studies in freely behaving rodents. This work uses microelectromechanical systems (MEMS) technology to design and fabricate a small and lightweight transducer capable of artifact‐free stimulation and simultaneous neural recording. Using the general‐purpose system, it can be observed that state‐dependent ultrasound neuromodulation of the prefrontal cortex increases rapid eye movement (REM) sleep and protects spatial working memory to REM sleep deprivation. The system will allow explorative studies in brain disease therapeutics and neuromodulation using ultrasound stimulation for widespread clinical adoption. Abstract : This work demonstrates a chronic, artifact‐free, and closed‐loop ultrasound stimulation system based on a capacitive micromachined ultrasound transducer for neuromodulation of the medial prefrontal cortex in mice. Stimulation during non‐rapid eye movement (NREM) sleep modulates REM sleep characteristics and short‐term spatial working memory. Neuroprotective effects of REM sleep are observed, which negates the adverse effects of REM sleep deprivation in spatial working memory.