Anti-diabetic activity of extract from Morus nigra L. twigs through activation of AMPK/PKC pathway in mice. (December 2022)

• Record Type: Journal Article
• Authors: Tang, Ping; Yang, Xinzhou; Chen, Huijian; Zhang, Ting; Tang, Hui; Pang, Kejian
• Is Part Of: Journal of functional foods. Volume 99(2022)
• Publication Date: 2022-12
• Subjects: Morus nigra L. -- Type 2 diabetes mellitus -- Glucose transporter 4 (GLUT4) -- Protein kinase C (PKC) -- AMP-activated protein kinase (AMPK); MNT-EA ethyl acetate extract of Morus nigra L. twigs -- T2DM type 2 diabetes mellitus -- GLUT4 glucose Transporter 4 -- AMPK AMP-activated protein kinase -- PKC protein kinase C -- IR insulin resistance -- HFD high-fat diet -- STZ streptozotocin -- HPLC high performance liquid chromatography -- α-MEM α-Minimum Essential Medium -- FBS fetal bovine serum -- INS insulin -- ELISA enzyme-linked immunosorbent assay -- FFA free fatty acid -- ASE accelerated solvent extraction -- IRAP insulin-regulated aminopeptidase -- GSVs glut4 storage vesicles -- VSVG vesicular stomatitis virus G -- OGTT oral glucose tolerance test -- ITT insulin tolerance test -- LDL-C low-density lipoprotein cholesterol -- HDL-C high-density lipoprotein cholesterol -- TC total cholesterol -- TG triglycerides -- HOMA-IR homeostasis model assessment of insulin resistance -- FBG fasting blood glucose -- HE hematoxylin and eosin -- SDS-PAGE sodium dodecyl sulphate-polyacrylamide gel electrophoresis -- AUC the area under the curves; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2022.105358
• Languages: English
• ISSNs: 1756-4646

Abstract

Graphical abstract: Highlights: The 21 main chemical components in MNT-EA were identified by UPLC-Q-TOF/MS. MNT-EA ameliorated a series of diabetes-related indices. MNT-EA improved the pathological changes in liver, adipose and pancreatic tissues. MNT-EA up-regulates AMPK and PKC pathways to regulate glucose metabolism in vivo . Abstract: Morus nigra L. is a traditional ethnomedicinal plants, which is used for the treatment of atherosclerosis, diabetes, hyperlipidemia, and hypertension. The aim of this study was to investigate the antidiabetic activity and potential mechanisms of ethyl acetate extract of Morus nigra L. twigs (MNT-EA). In L6 cells, MNT-EA significantly upregulated glucose uptake, and promoted GLUT4 translocation and expression through AMPK and PKC signaling pathway. In vivo, diabetic mice treated with MNT-EA for 4 weeks improved hyperglycemia, lipid metabolism disorders and insulin resistance. According to histopathological examination, MNT-EA attenuated hepatic steatosis, adipocyte hypertrophy, and islet lesions in diabetic mice. In addition, MNT-EA upregulated p-AMPK, p-PKC, and GLUT4 expression in liver, adipose, and skeletal muscle tissues, thereby enhancing glucose uptake and improving diabetic symptoms. It is the first time to demonstrate that MNT-EA exerted anti-diabetic effects through activation of AMPK/PKC/GLUT4 signaling pathway, providing a basis for the development of new anti-diabetic drugs.