3D‐Engineered Scaffolds to Study Microtubes and Localization of Epidermal Growth Factor Receptor in Patient‐Derived Glioma Cells. Issue 49 (7th October 2022)

• Record Type: Journal Article
• Authors: Barin, Nastaran; Balcioglu, Hayri E.; de Heer, Iris; de Wit, Maurice; Lamfers, Martine L. M.; van Royen, Martin E.; French, Pim J.; Accardo, Angelo
• Is Part Of: Small. Volume 18:Issue 49(2022)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2022-10-07
• Subjects: 2‐photon polymerization -- 3D cell culture -- brain cancer -- epidermal growth factor receptor -- microtubes; Nanotechnology -- Periodicals; Nanoparticles -- Periodicals; Microtechnology -- Periodicals; 620.5
• DOI: 10.1002/smll.202204485
• Languages: English
• ISSNs: 1613-6810

Abstract

Abstract: A major obstacle in glioma research is the lack of in vitro models that can retain cellular features of glioma cells in vivo. To overcome this limitation, a 3D‐engineered scaffold, fabricated by two‐photon polymerization, is developed as a cell culture model system to study patient‐derived glioma cells. Scanning electron microscopy, (live cell) confocal microscopy, and immunohistochemistry are employed to assess the 3D model with respect to scaffold colonization, cellular morphology, and epidermal growth factor receptor localization. Both glioma patient‐derived cells and established cell lines successfully colonize the scaffolds. Compared to conventional 2D cell cultures, the 3D‐engineered scaffolds more closely resemble in vivo glioma cellular features and allow better monitoring of individual cells, cellular protrusions, and intracellular trafficking. Furthermore, less random cell motility and increased stability of cellular networks is observed for cells cultured on the scaffolds. The 3D‐engineered glioma scaffolds therefore represent a promising tool for studying brain cancer mechanobiology as well as for drug screening studies. Abstract : The development of a biomimetic in vitro 3D‐engineered glioma culture model fabricated by two‐photon polymerization and cultured with patient‐derived cells is reported. Compared to 2D cultures, the 3D model more closely resembles the in vivo glioma cellular morphology, has a more stable cellular network, and allows better monitoring of individual cells, cellular protrusions, and intracellular trafficking.