Effect of dietary replacement of fish meal by poultry by-product meal on the growth performance, immunity, and intestinal health of juvenile red swamp crayfish, procambarus clarkia. Issue 131 (December 2022)

• Record Type: Journal Article
• Authors: Yang, Mengxi; Guo, Xiaorui; Chen, Tuo; Li, Peng; Xiao, Tiaoyi; Dai, Zhenyan; Hu, Yi
• Is Part Of: Fish & shellfish immunology. Issue 131(2022)
• Page Start: 381
• Page End: 390
• Publication Date: 2022-12
• Subjects: Poultry by-product meal -- Immune response -- Antioxidant capacity -- Intestinal morphology -- Intestinal microbiota -- Procambarus clarkia; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2022.10.025
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: The present study was conducted to investigate the dietary replacement of fish meal with poultry by-product meal (PBM) on the growth performance, immunity, antioxidant properties, and intestinal health of red swamp crayfish ( Procambarus clarkia ). A diet containing 20% fish meal (FM) and complex plant ingredients as the main protein resources was set as the FM group (crude protein 32%, crude lipid 6%). Four diets replacing 25%, 50%, 75%, and 100% fish meal of the FM diet with PBM were set as the PBM25, PBM50, PBM75, and PBM100 groups, respectively. Compared to the FM group, the PBM100 diet significantly decreased growth performance and feed utilization of crayfish, while markedly increasing the activity of serum aspartate aminotransferase. The immune response was depressed in crayfish fed the PBM100 diet as the activities of serum lysozyme and phenoloxidase, gene expression of anti-lipopolysaccharide factors ( alf ), cyclophilin A ( cypa ), crustin, and hemocyanin-1 ( hep - 1 ) in hepatopancreas were remarkably decreased. The activities of antioxidases and expression of antioxidant-relevant genes in the hepatopancreas were not influenced by PBM inclusion. Crayfish fed different diets exhibited no obvious symptoms of enteritis, but the PBM100 diet destructed intestinal morphology by significantly decreasing the average length of longitudinal ridges. The α-diversity and overall community structure were not significantly influenced but variations were found in the relative abundance of some genera by PBM inclusion. In summary, CAP could successfully replace 75% dietary FM in a basal diet containing 20% fish meal, while higher CAP level compromised growth performance, immunity, and intestinal histology of crayfish. Highlights: PBM replacing 75% FM did not reduce growth performance and intestinal morphology of crayfish. PBM replacing 75% FM did not depress immunity and hepatopancreas health of crayfish. PBM inclusion showed no adverse effects on antioxidant properties of crayfish. Overall intestinal microbial community structure was not altered by PBM inclusion. PBM changed abundances of some intestinal bacteria genus of crayfish.