Armillariella tabescens methanol extract ameliorates ulcerative colitis via inhibiting TLR4/NF-κB and NLRP3 activation and mediating intestinal barrier integrity. (December 2022)

• Record Type: Journal Article
• Authors: Zhang, Wen-Na; Li, Yuan-Yuan; Xu, Jia-Jia; Shi, Lu-Lu; Chen, Lei; Lu, Yong-Ming; Wu, Qing-Xi; Luo, Jian-Guang; Chen, Yan
• Is Part Of: Journal of functional foods. Volume 99(2022)
• Publication Date: 2022-12
• Subjects: Armillariella tabescens -- Ulcerative colitis -- Tight junction proteins -- TLR4/NF-κB -- NLRP3; AB-PAS alcian blue and periodic acid-schiff -- AME Armillariella tabescens methanol extract -- ANOVA one-way analysis of variance -- DSS dextran sulfate sodium -- DAI disease activity index -- ELISA enzyme-linked immunosorbent assay -- H&E hematoxylin and eosin -- HPLC high performance liquid chromatography -- HRMS high resolution mass spectroscopy -- IBD inflammatory bowel diseases -- LPS lipopolysaccharides -- MPO myeloperoxidase -- NLRP3 NOD-like receptor pyrin domain-containing protein 3 -- UC ulcerative colitis -- SASP salazosulfapyridine -- SEM standard error of mean -- TJs tight junctions -- TLR4 toll-like receptor 4 -- TNF-α tumor necrosis factor-α; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2022.105301
• Languages: English
• ISSNs: 1756-4646

Abstract

Graphical abstract: Highlights: Armillariella tabescens methanol extract (AME) significantly attenuated UC symptoms, suppressed the activity of myeloperoxidase (MPO), decreased the levels of LPS, TNF- α, IL-1 β, IL-18, and increased the level of IL-10 in serum. AME could improve the expression of tight junction proteins ZO-1, occludin and claudin-1. AME could inhibit the activation of TLR4/NF-κB and NLRP3 signaling pathways in colon tissue. Abstract: Armillariella tabescens is a traditional Chinese medicinal and edible fungus which holds importance for treatment of gastrointestinal disorders. This study was aimed to evaluate the effect of methanol extract of A. tabescens (AME) in dextran sulfate sodium (DSS) induced ulcerative colitis (UC). The results showed that AME significantly attenuated UC symptoms, suppressed the activity of myeloperoxidase (MPO), decreased the levels of LPS, TNF- α, IL-1 β, IL-18, and increased the level of IL-10 in serum. Besides, AME could improve the expression of tight junction proteins ZO-1, occludin and claudin-1. Moreover, AME inhibited the activation of TLR4/NF-κB and NLRP3 signaling pathways in colon tissue. In addition, eight compounds were identification in AME by high performance liquid chromatography (HPLC)-high resolution mass spectroscopy (HRMS). Therefore, this study indicated that AME was potential to be further developed into a suitable functional food additive or promising therapeutic agent for UC treatment.