Preparation and properties of porous biphasic calcium phosphate/bioactive glass composite scaffolds for biomedical applications. (December 2022)

• Record Type: Journal Article
• Authors: Monmaturapoj, Naruporn; Uanlee, Theerawat; Nampuksa, Katanchalee; Kasiwat, Anchittha; Makornpan, Chalumkwan
• Is Part Of: Materials today communications. Volume 33(2022)
• Publication Date: 2022-12
• Subjects: Bioactive glass -- 45S5 -- Biphasic calcium phosphate -- Hydroxyapatite -- Tricalcium phosphate -- Bioactivity; Materials science -- Periodicals; 620.11
• DOI: 10.1016/j.mtcomm.2022.104993
• Languages: English
• ISSNs: 2352-4928

Abstract

Abstract: Porous biphasic calcium phosphate (BCP) scaffolds were prepared on addition of 45S5 bioactive glass (BG) in different proportions. Porous scaffolds were fabricated using a polymer foaming technique for investigating their properties. The obtained scaffolds were characterized by X-ray diffraction (XRD) and scanning electron microscopy (SEM) to determine the phase formation and morphology, respectively. The physical properties and compressive strength were also measured. MC3T3-E1 osteoblast-like cell was used to analyses cell proliferation on the scaffold samples by Alamar Blue® assay and cell morphology was revealed by SEM. Antibacterial activity and In vitro bioactivity in simulated body fluid (SBF) solution were conducted to detect the percentage of bacterial reduction and to determine bone bonding ability through the formation of apatite layer of the scaffolds, respectively. From XRD patterns, a higher degree of HA transformation to β-TCP was observed with increasing of BG amount. The density, shrinkage, and compressive strength of materials also raised up with BG amount. All BCP scaffolds containing BG obtained 99% antibacterial activity better than the original BCP. BCP with 7.5% BG performed the strongest scaffold couple with the lowest in cell viability within 14 days. Furthermore, the cauliflower-like morphology of apatite layer formed on all BCP scaffolds reinforced with BG surfaces after 14 days immersion in the SBF. Therefore, bioactive glass played an important role in inducing the mechanical, antibacterial properties and in vitro bioactivity of biphasic calcium phosphate porous scaffolds which is more suitable to be used for biomedical applications than the original BCP. Graphical Abstract: ga1 Highlights: Bioactive glass induced a phase transformation and enhanced densification of biphasic calcium phosphate composite. All composite scaffolds exhibited a superior antibacterial property and in vitro bioactivity than the original scaffolds. Composite scaffolds demonstrated good cell adhesion and proliferation after cultured the osteoblast cells for 14 days. The porous composite scaffold showed potential for use as bone substitution by the addition of bioactive glass.