MiR319 and its target TCP4 involved in plant architecture regulation in Brassica napus. (January 2023)

• Record Type: Journal Article
• Authors: Lu, Hongchen; Chen, Li; Du, Mengjie; Lu, Haiqin; Liu, Jie; Ye, Shenhua; Tao, Baolong; Li, Rihui; Zhao, Lun; Wen, Jing; Yi, Bin; Tu, Jinxing; Fu, Tingdong; Shen, Jinxiong
• Is Part Of: Plant science. Volume 326(2023)
• Publication Date: 2023-01
• Subjects: Brassica napus -- MicroRNAs -- Plant architecture -- Shoot apical meristem -- MiR319; Botany -- Periodicals; Botanique -- Périodiques; 580
• DOI: 10.1016/j.plantsci.2022.111531
• Languages: English
• ISSNs: 0168-9452

Abstract

Abstract: Plant architecture is a collection of genetically controlled crop productivity and adaptation. MicroRNAs (miRNAs) have been proved to function in various biological processes, but little is known about how miRNA regulates plant architecture in rapeseed ( Brassica napus L.). In this study, four small RNA libraries and two degradome libraries from shoot apex of normal and rod-like plants were sequenced. A total of 639 miRNA precursors and 16 differentially expressed miRNAs were identified in this study. In addition, 322 targets were identified through degradome sequencing. Among them, 14 targets were further validated via RNA ligase-mediated 5′ rapid amplification of cDNA ends. Transgenic approach showed that increased TCP4 activity in Arabidopsis resulted in premature onset of maturation and reduced plant size along with early flowering and shortened flowering time. miR319-OE lines in Brassica napus exhibited serrated leaves and abnormal development of shoot apical meristem (SAM), which led to the deformed growth of stem and reduced plant height. In conclusion, our study lays the foundation for elucidating miRNA regulate plant architecture and provides new insight into the miR319/ TCP4 module regulates plant architecture in rapeseed. Highlights: First integrated microRNA and degradome sequencing to investigate plant architecture regulation in Brassica napus . 322 transcripts were predicted to be degraded by 25 known and 13 novel miRNAs. We identified 10 known miRNAs and 6 novel miRNAs related to plant architecture development. The appropriate expression level of miR319 and its target TCP4 is significant for plant development. Our work provides new insight into the miR319/ TCP4 module regulates plant architecture in rapeseed.