Myticofensin, a novel antimicrobial peptide family identified from Mytilus coruscus. Issue 131 (December 2022)

• Record Type: Journal Article
• Authors: Liu, Lu; He, Menglan; Yang, Zongxin; Wang, Haodong; Zhang, Xiaolin; He, Jianyu; Buttino, Isabella; Qi, Pengzhi; Yan, Xiaojun; Liao, Zhi
• Is Part Of: Fish & shellfish immunology. Issue 131(2022)
• Page Start: 817
• Page End: 826
• Publication Date: 2022-12
• Subjects: Mytilus coruscus -- Antimicrobial peptide -- Myticofensin -- Expression profile -- Chemical synthesis; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2022.10.057
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: In this study, seven transcripts representing a novel antimicrobial peptide (AMP) family with structural features similar to those of arthropod defensins were identified from Mytilus coruscus . These novel defensins from the Mytilus AMP family were named myticofensins. To explore the possible immune-related functions of these myticofensins, we examined their expression profiles in different tissues and larval stages, as well as in three immune-related tissues under the threat of different microbes. Our data revealed that the seven myticofensins had relatively high expression levels in immune-related tissues. Most myticofensins were undetectable, or had low expression levels, in different larval mussel stages. Additionally, in vivo microbial challenges significantly increased the expression levels of myticofensins in M. coruscus hemocytes, gills, and digestive glands, showing different immune response patterns under challenges from different microbes. Our data indicates that different myticofensins may have different immune functions in different tissues. Furthermore, peptide sequences corresponding to the beta-hairpin, alpha-helix, and N-terminal loop of myticofensin were synthesized and the antimicrobial activities of these peptide fragments were tested. Our data confirms the diversity of defensins in Mytilus and reports the complex regulation of these defensins in the mussel immune response to different microbes in immune-related tissues. The immune system of Mytilus has been studied for years as they are a species with strong environmental adaptations. Our data can be regarded as a step forward in the study of the adaptation of Mytilus spp. to an evolving microbial world. Highlights: Seven novel antimicrobial peptides named myticofensin were identified from M. coruscus . The expression profiles of the myticofensin genes were determined. Myticofensins showed different responses to different microbial challenges in vivo . The possible active sites were verified for several myticofensins.