Alleviating effects of essential oil from Artemisia vulgaris on enteritis in zebrafish via modulating oxidative stress and inflammatory response. Issue 131 (December 2022)

• Record Type: Journal Article
• Authors: Meng, Rui; Wu, Shanshan; Chen, Jianjie; Cao, Jinling; Li, Lijuan; Feng, Cuiping; Liu, Jingyu; Luo, Yongju; Huang, Zhibing
• Is Part Of: Fish & shellfish immunology. Issue 131(2022)
• Page Start: 323
• Page End: 341
• Publication Date: 2022-12
• Subjects: Essential oil from Artemisia vulgaris -- Zebrafish -- Enteritis -- Oxidative stress -- Inflammatory response; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2022.10.010
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: Artemisia vulgaris ( A. vulgaris ) is a traditional Chinese medicine widely distributed in China and contains many bioactive compounds with pharmacological effects. However, the anti-inflammatory effects and mechanism of essential oil from A. vulgaris on enteritis in fish are still unclear. In this study, in order to elucidate the underlying mechanism of essential oil from A. vulgaris on zebrafish enteritis, zebrafish were used for establishing animal models to observe the histopathological changes of intestines, determine the activities of immune-related enzymes and oxidative stress indicators, and the mRNA expression of genes in MyD88/TRAF6/NF-KB signaling pathways. The results showed that different doses of A. vulgaris essential oil could effectively alleviate zebrafish enteritis in a dose- and time-dependent manner by improving the intestinal histopathological damage, decreasing the intestinal oxidative stress, repairing the intestinal immune ability, changing the expression levels of IL-1β, IL-10 and genes in MyD88/TRAF6/NF-κB pathway. In addition, co-treatment with oxazolone and MyD88 inhibitor could alleviate the morphological damage, the induction of oxidative stress, and the levels of immune-related enzymes and the mRNA expression of genes in MyD88/TRAF6/NF-κB signaling pathway. Moreover, essential oil from A. vulgaris had more significantly therapeutic effects on enteritis of male zebrafish than that of female zebrafish. This result will clarify the therapeutic effect and anti-inflammatory mechanism of essential oil from A. vulgaris on zebrafish enteritis, and provide a theoretical basis for further research on the rationality of A. vulgaris to replace feed antibiotics. Graphical abstract: Image 1 Highlights: A. vulgaris essential oil could improve the intestinal histopathological damage. A. vulgaris essential oil could decrease oxidative stress and repair immune capacity of gut. It could change the expression levels of genes in MyD88/TRAF6/NF-κB pathway. It had better therapeutic effects on enteritis of males than that of females. It could effectively alleviate zebrafish enteritis.