The changing chromatome as a driver of disease: A panoramic view from different methodologies. (9th November 2020)

• Record Type: Journal Article
• Authors: Espejo, Isabel; Di Croce, Luciano; Aranda, Sergi
• Is Part Of: BioEssays. Volume 42:Number 12(2020:Dec.)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2020-11-09
• Subjects: chromatin -- gene regulation -- proteome; Molecular biology -- Periodicals; Cytology -- Periodicals; Developmental biology -- Periodicals; 572.8
• DOI: 10.1002/bies.202000203
• Languages: English
• ISSNs: 0265-9247

Abstract

Abstract: Chromatin‐bound proteins underlie several fundamental cellular functions, such as control of gene expression and the faithful transmission of genetic and epigenetic information. Components of the chromatin proteome (the "chromatome") are essential in human life, and mutations in chromatin‐bound proteins are frequently drivers of human diseases, such as cancer. Proteomic characterization of chromatin and de novo identification of chromatin interactors could, thus, reveal important and perhaps unexpected players implicated in human physiology and disease. Recently, intensive research efforts have focused on developing strategies to characterize the chromatome composition. In this review, we provide an overview of the dynamic composition of the chromatome, highlight the importance of its alterations as a driving force in human disease (and particularly in cancer), and discuss the different approaches to systematically characterize the chromatin‐bound proteome in a global manner. Abstract : Chromatin‐bound proteins regulate gene expression, replicate and repair DNA, and transmit epigenetic information. Several human diseases are highly influenced by alterations in the chromatin‐bound proteome. Thus, biochemical approaches for the systematic characterization of the chromatome could contribute to identifying new regulators of cellular functionality, including those that are relevant to human disorders.