Design and performance of a dark‐field probe with confocal Raman spectroscopy for ophthalmic applications. (5th May 2021)

• Record Type: Journal Article
• Authors: Zhang, Shuo; Erckens, Roel J.; Jongsma, Franciscus H.M.; Webers, Carroll A.B.; Berendschot, Tos T.J.M.
• Is Part Of: Journal of Raman spectroscopy. Volume 52:Number 7(2021)
• Page Start: 1371
• Page End: 1375
• Publication Date: 2021-05-05
• Subjects: aqueous humor -- dark‐field illumination -- ocular drug -- Raman spectroscopy; Raman spectroscopy -- Periodicals; 535.846
• DOI: 10.1002/jrs.6125
• Languages: English
• ISSNs: 0377-0486

Abstract

Abstract: A dark‐field ophthalmic contact probe for analysis of aqueous humor (AH) was designed by ray tracing and constructed in quartz glass for experimental use as a prototype probe in a confocal Raman spectroscopic setup. The misalignment tolerance of this probe was theoretically evaluated, whereas the optical performance was experimentally evaluated by a concentration curve of the ocular drug phenylephrine HCl in an artificial eye model. The results indicated a ninefold improvement in misalignment tolerance and a 36‐fold improvement in the signal‐to‐noise ratio at the phenylephrine HCl 1002 cm −1 peak compared with a previous design. The prototype showed a good linear fit with the phenylephrine HCl concentration, with an R 2 of 0.996. Phenylephrine HCl concentration down to 0.1% is detectable. Abstract : A dark‐field ophthalmic probe for analysis of aqueous humor was designed and constructed for experimental use in a confocal Raman spectroscopic setup. The results indicated a ninefold improvement in misalignment tolerance and a 36‐fold improvement in the signal‐to‐noise ratio when measuring an ocular drug (phenylephrine HCl) compared with a previous design. The prototype showed a good linear fit with the drug concentration, with an R 2 of 0.996 and a sensitivity detection limit down to 0.1% phenylephrine HCl.