Genotyping of enteroviruses and human parechoviruses highlights their diversity in Northwest Ethiopia. Issue 12 (20th March 2020)

• Record Type: Journal Article
• Authors: Gelaw, Aschalew; Pietsch, Corinna; Tigabu, Zemene; Liebert, Uwe G.
• Is Part Of: Journal of medical virology. Volume 92:Issue 12(2020)
• Page Start: 3007
• Page End: 3015
• Publication Date: 2020-03-20
• Subjects: children -- diarrhea -- enteroviruses -- genetic diversity -- human parechovirus -- molecular epidemiology -- Northwest Ethiopia; Virology -- Periodicals; 616
• DOI: 10.1002/jmv.25765
• Languages: English
• ISSNs: 0146-6615

Abstract

Abstract: Enteroviruses (EVs) and human parechoviruses (HPeVs) infections are associated with various forms of disease, including gastroenteritis. As information on the molecular epidemiology of these viruses is limited in Ethiopia, the genetic diversity of EV and HPeV was investigated in the Northwestern part of the country. Of the total 450 stool samples obtained from infants and young children with diarrhea, 157 (34.9%) were positive for EV and 49 (10.9%) for HPeV RNA when tested by real‐time reverse transcription polymerase chain reaction. Genotyping was performed by sequencing of the EV VP1 gene and the HPeV VP3/VP1 gene, respectively. Genotyping of EV was successful in 118 samples. Thereof, 82 (69.5%) belonged to non‐polio EVs as a broad range of genotypes within species C, B, and A. Sabin polioviruses were found in 36 cases. HPeV sequences were also heterogeneous with a relative dominance of genotype 3. In conclusion, diverse EV and HPeV genotypes were found cocirculating in Northwest Ethiopia. The findings highlight the importance of continuous surveillance of these viruses in Ethiopia.