Pseudoshikonin I enhances osteoblast differentiation by stimulating Runx2 and Osterix. Issue 1 (31st July 2017)

• Record Type: Journal Article
• Authors: Choi, You Hee; Han, Younho; Jin, Sun Woo; Lee, Gi Ho; Kim, Geum Soog; Lee, Dae Young; Chung, Young Chul; Lee, Kwang Youl; Jeong, Hye Gwang
• Is Part Of: Journal of cellular biochemistry. Volume 119:Issue 1(2018)
• Page Start: 748
• Page End: 757
• Publication Date: 2017-07-31
• Subjects: osteoblast differentiation -- osterix -- pseudoshikonin I -- Runx2; Cytochemistry -- Periodicals; 572
• DOI: 10.1002/jcb.26238
• Languages: English
• ISSNs: 0730-2312

Abstract

Abstract: Pseudoshikonin I (PSI), a novel biomaterial isolated from Lithospermi radix, has been recognized as an herbal medicine for the treatment of infectious and inflammatory diseases. Bone remodeling maintains a balance through bone resorption (osteoclastogenesis) and bone formation (osteoblastogenesis). Bone formation is generally attributed to osteoblasts. However, the effects of PSI on the bone are not well known. In this study, we found that the ethanol extracts of PSI induced osteoblast differentiation by increasing the expression of bone morphogenic protein 4 (BMP 4). PSI positively regulates the transcriptional expression and osteogenic activity of osteoblast‐specific transcription factors such as Runx2 and Osterix. To identify the signaling pathways that mediate PSI‐induced osteoblastogenesis, we examined the effects of serine‐threonine kinase inhibitors that are known regulators of Osterix and Runx2. PSI‐induced upregulation of Osterix and Runx2 was suppressed by treatment with AKT and PKA inhibitors. These results suggest that PSI enhances osteoblast differentiation by stimulating Osterix and Runx2 via the AKT and PKA signaling pathways. Thus, the activation of Runx2 and Osterix is modulated by PSI, thereby demonstrating its potential as a treatment target for bone disease. Abstract : PSI enhances osteoblast differentiation by influencing transcript and protein levels of the osteoblast transcription factors Osterix and Runx2. PSI regulates Runx2 and Osterix expression through PKA and AKT signaling in osteoblast differentiation.