Promotive effect of Talin‐1 protein on gastric cancer progression through PTK2‐PXN‐VCL‐E‐Cadherin‐CAPN2‐MAPK1 signaling axis. Issue 12 (20th September 2020)

• Record Type: Journal Article
• Authors: Yan, Hongzhu; Guo, Min; Zou, Jue; Xiao, Feng; Yi, Lina; He, Ying; He, Bosheng
• Is Part Of: Journal of clinical laboratory analysis. Volume 34:Issue 12(2020)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2020-09-20
• Subjects: gastric cancer -- mechanism -- MKN‐45 cells -- signaling axis -- talin‐1; Diagnosis, Laboratory -- Periodicals; Medical laboratory technology -- Periodicals; 616
• DOI: 10.1002/jcla.23555
• Languages: English
• ISSNs: 0887-8013

Abstract

Abstract: Objective: Our research group was aim to explore the molecular mechanism of Talin‐1 protein affecting gastric cancer progression through PTK2‐PXN‐VCL‐E‐Cadherin‐CAPN2‐MAPK1 signal axis. Methods: 12 cases of patients with gastric cancer in this hospital from 2018 to 2019 were collected. Immunohistochemistry assay and Western blotting were used to detect the expression of Talin‐1, PXN, E‐Cadherin, CAPN2, MAPK1 protein in gastric cancer tissue. Cell migration and invasion were measured by Transwell. Results: The results showed that the expression levels of protein Talin‐1, PXN and MAPK1 in gastric cancer tissues were significantly higher than that in normal tissue. The number of cell adhesion in the model group was significantly lower than that in the normal group. However, the cell adhesion number in ov‐TLN1 was the highest. Transwell results showed that TLN1 could accelerate the migration and invasion abilities of gastric cancer MKN‐45 cells. Moreover, Western blotting showed that protein Talin‐1, PXN, E‐Cadherin, CAPN2, MAPK1 in model group all increased compared with normal group. Conclusion: It indicated that talin‐1 protein influenced the development of gastric cancer through PTK2‐PXN‐VCL‐E‐Cadherin‐CAPN2‐MAPK1 signal axis. Abstract : Gastric cancer is one of the most common gastrointestinal malignancies. Adhesion and invasion of gastric cancer cells are related to various signaling pathways in vivo, among which integrin‐activated adhesion kinase‐mediated signaling pathway is an important pathway for tumor invasion and metastasis. Our research group aim was to explore the molecular mechanism of talin‐1 protein affecting gastric cancer progression through PTK2‐PXN‐VCL‐E‐Cadherin‐CAPN2‐MAPK1 signal axis. The results showed that the expression levels of protein talin‐1, PXN, and MAPK1 in gastric cancer tissues were significantly higher than that in normal tissue. The number of cell adhesion in the model group was significantly lower than that in the normal group. However, the cell adhesion number in ov‐TLN1 was the highest. Transwell showed that TLN1 can accelerate the migration and invasion abilities of gastric cancer MKN‐45 cells. Moreover, Western blotting showed that protein talin‐1, PXN, E‐Cadherin, CAPN2, MAPK1 in model group all increased compared with normal group. It indicated that talin‐1 protein influenced the development of gastric cancer through PTK2‐PXN‐VCL‐E‐Cadherin‐CAPN2‐MAPK1 signal axis.