Pyrophosphate as substrate for alkaline phosphatase activity: A convenient flow‐injection chemiluminescence assay. (21st April 2017)

• Record Type: Journal Article
• Authors: Zhang, Qingfeng; Zhang, Cuiyun; Yang, Meiding; Yu, Donghong; Yu, Cong
• Is Part Of: Luminescence. Volume 32:Number 7(2017)
• Page Start: 1150
• Page End: 1156
• Publication Date: 2017-04-21
• Subjects: alkaline phosphatase -- chemiluminescence -- pyrophosphate; Luminescence -- Periodicals; Bioluminescence -- Periodicals; Chemiluminescence -- Periodicals; Luminescence -- Periodicals; 535.35
• DOI: 10.1002/bio.3302
• Languages: English
• ISSNs: 1522-7235

Abstract

Abstract: A sensitive and convenient flow‐injection chemiluminescence (FI‐CL) turn‐on assay for alkaline phosphatase (ALP) activity without any label and synthesis is developed. Cu 2+ can catalyze the luminol–H2 O2 CL reaction. Pyrophosphate (PPi) can chelate Cu 2+ and therefore the Cu 2+ ‐mediated luminol‐H2 O2 CL reaction is inhibited. The addition of ALP can catalyze the hydrolysis of PPi into phosphate ions, Cu 2+ is released and the chemiluminescence recovers. A detection limit of 1 mU/mL ALP is obtained.