CD4-1 and CD8α T lymphocytes subsets in spotted sea bass (Lateolabrax maculatus) and comparison on antigenicity of T lymphocytes subsets in other three marine fish species. Issue 131 (December 2022)

• Record Type: Journal Article
• Authors: Jiang, Xiaoyu; Xing, Jing; Tang, Xiaoqian; Sheng, Xiuzhen; Chi, Heng; Zhan, Wenbin
• Is Part Of: Fish & shellfish immunology. Issue 131(2022)
• Page Start: 487
• Page End: 497
• Publication Date: 2022-12
• Subjects: Molecular cloning -- Lateolabrax maculatus -- CD4 -- CD8 -- T lymphocytes -- Keyhole limpet hemocyanin (KLH) -- Cross-reactivity; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2022.10.004
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: CD4 and CD8 molecules play an important role in the identification of T lymphocytes, and diverse among fish species. In this study, CD4-1 and CD8α gene of spotted sea bass ( Lateolabrax maculatus ) were cloned, polyclonal antibodies against CD4-1 (CD4-1 pAbs) and CD8α (CD8α pAbs) were produced, respectively. And the variations in CD4-1 + and CD8α + T-lymphocytes in spotted sea bass and the cross-reactivity with leukocytes in pearl gentian grouper ( Epinephelus fuscoguttatus x E. lanceolatus ), schlegel's black rockfish ( Sebastes schlegelii ) and flounder ( Paralichthys olivaceus ) were investigated using CD4-1 pAbs and CD8α pAbs. The results showed that CD4-1 molecule ORF was 1413 bp and CD8α was 690 bp, both molecules are transmembrane glycoproteins with high amino acid homology to grouper. The CD4-1 pAbs specifically recognized both the CD4-1 recombinant and natural proteins, as does the CD8α pAbs to CD8α molecule, and no cross-reactivity between the two antibodies. CD4-1 + and CD8α + T lymphocytes were detected in peripheral blood, spleen and head kidney leukocytes in spotted sea bass. In cross-reactivity assay with other three fish, CD4-1 pAbs could recognize the lymphocytes from pearl gentian grouper and schlegel's black rockfish, both with highest proportions in the spleen leukocytes, 5.3 ± 0.4% and 2.6 ± 0.3%, respectively, and CD8α pAbs could only recognize the lymphocytes in pearl gentian grouper, and no cross-reactivities to lymphocytes of flounder. These data suggested that the CD4-1 and CD8α molecules varied by fish species in the genes features and antigenicity, which might result in the diversities of T lymphocytes subpopulations. This will be a key to elucidating the classification and evolution of T lymphocytes in fish. Highlights: Both CD4-1 and CD8α gene of spotted sea bass were homologous most to grouper. The pAbs recognize CD4-1/CD8α, respectively, didn't cross-reacted to each other. The highest proportions of CD4-1/CD8α + cells were in HKLs and SPLs, respectively. The pAbs cross-reacted to lymphocytes in grouper and rockfish, not in flounder.