Vitamins and their derivatives synergistically promote hair shaft elongation ex vivo via PlGF/VEGFR-1 signalling activation. Issue 1 (October 2022)

• Record Type: Journal Article
• Authors: Hu, Liuying; Kimura, Shun; Haga, Masatoshi; Kashiwagi, Sayo; Takagi, Kyoko; Shimizu, Takashi; Ishii, Tsuyoshi; Ohyama, Manabu
• Is Part Of: Journal of dermatological science. Volume 108:Issue 1(2022)
• Page Start: 2
• Page End: 11
• Publication Date: 2022-10
• Subjects: QOL quality of life -- Vits vitamins and their derivatives -- PEE panthenyl ethyl ether -- PH pyridoxine hydrochloride -- TA tocopherol acetate -- VEGFR-1 vascular endothelial growth factor receptor-1 -- Plgf placental growth factor -- Igf1 insulin-like growth Factor 1 -- Hgf hepatocyte growth factor -- Fgf7 fibroblast growth Factor 7 -- Vegfa vascular endothelial growth Factor a -- Pdgfa platelet-derived growth factor subunit a -- MPHL male-pattern hair loss -- FPHL female-pattern hair loss -- HF hair follicle -- DP dermal papilla -- hDPCs human dermal papilla cells -- HFKCs human follicular keratinocytes -- PCA principal component analysis -- GO Gene Ontology; Vitamin -- Hair shaft elongation -- Dermal papilla -- Hair matrix cell -- PlGF -- VEGFR-1; Dermatology -- Periodicals; Skin Diseases -- Periodicals; Dermatologie -- Périodiques; 616.5005
• DOI: 10.1016/j.jdermsci.2022.09.003
• Languages: English
• ISSNs: 0923-1811

Abstract

Abstract: Background: Although vitamins or their derivatives (Vits), such as panthenyl ethyl ether, tocopherol acetate, and pyridoxine, have been widely used in topical hair care products, their efficacy and mode of action have been insufficiently studied. Objective: To elucidate the biological influence of Vits on hair follicles and determine the underlying mechanisms. Methods: A mouse vibrissa hair follicle organ culture model was utilized to evaluate the effects of Vits on hair shaft elongation. Gene and protein expression analyses and histological investigations were conducted to elucidate the responsible mechanisms. A human hair follicle cell culture was used to assess the clinical relevance. Results: In organ culture models, the combination of panthenyl ethyl ether, tocopherol acetate, and pyridoxine (namely, PPT) supplementation significantly promoted hair shaft elongation. PPT treatment enhanced hair matrix cell proliferation by 1.9-fold compared to controls, as demonstrated by Ki67-positive immunoreactivity. PPT-treated mouse dermal papillae exhibited upregulated Placental growth factor (Plgf) by 1.6-fold compared to controls. Importantly, the addition of PlGF neutralizing antibodies to the ex vivo culture diminished the promotive effect on hair growth and increase in VEGFR-1 phosphorylation achieved by PPT. A VEGFR-1 inhibitor also inhibited the promotion of hair growth. Microarray analysis suggested synergistic summation of individual Vits' bioactivity, putatively explaining the effect of PPT. Moreover, PPT increased PlGF secretion in cultured human dermal papilla cells. Conclusion: Our findings suggested that PPT promoted hair shaft elongation by activating PlGF/VEGFR-1 signalling. The current study can shed light on the previously underrepresented advantage of utilizing Vits in hair care products. Highlights: The combination of three vitamins and their derivatives (PPT) promoted mouse vibrissae hair shaft elongation ex vivo . PPT promoted hair matrix cell proliferation in mouse vibrissae ex vivo . PlGF was upregulated by PPT in mouse vibrissa dermal papilla cells ex vivo . PPT-induced hair shaft elongation was PlGF/VEGFR1 signalling pathway dependent. PlGF expression is increased by PPT in primary cultured human dermal papilla cells.