The flavonoids extract from Okra flowers protects against DSS-induced colitis via regulating NF-κB signaling pathway and gut microbiota. (December 2022)

• Record Type: Journal Article
• Authors: Huang, Xiaoyi; Li, Xingjie; Deng, Yuanle; Zhou, Ting; Chen, Tao; Wu, Shouxun; Xia, Rui; Kang, Yuhong; Yin, Wenya
• Is Part Of: Journal of functional foods. Volume 99(2022)
• Publication Date: 2022-12
• Subjects: AFE -- Flavonoids -- Inflammatory bowel disease -- Gut microbiota -- NF-κB signaling pathway; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2022.105335
• Languages: English
• ISSNs: 1756-4646

Abstract

Graphical abstract: Highlights: Four flavonoids in AFE were clearly quantified using HPLC and standards. AFE inhibits DSS-induced abnormal NF-κB pathway activation in vivo and in vitro . Akkermansia is key for AFE to alleviate intestinal inflammation. AFE exerted an antioxidant effect as a ROS scavenger in vitro . Abstract: Okra flower is an untapped flavonoid-rich resource, and flavonoids from it (AFE) have been demonstrated intestinal protective potential due to anti-colorectal cancer properties, but other intestinal-related effects remain to be explored. Here, we found that AFE, as a safe natural product, effectively alleviate 2.5% dextran sulfate sodium (DSS)-induced intestinal inflammation in C57BL/6N and DSS-induced injury of human intestinal epithelial cells NCM460. AFE maintained colon integrity by promoting the levels of tight junction proteins, ZO-1 and Occludin, and modulating the NF-κB signaling pathway to prevent further tissue damage. Meanwhile, gut microbiota was involved in the protective effect of AFE in vivo, with Akkermansia making a major contribution. Moreover, AFE exhibited similar inhibitory effects on the NF-κB pathway in vitro and exerted antioxidant effects as a ROS scavenger by regulating the Nrf2 signaling pathway. In summary, this study demonstrated the potential of AFE in restoring intestinal health in vitro and in vivo .