An effective restoration of one-carbon metabolism in folate-deficient mice with a high-folate corn inbred line. (December 2022)

• Record Type: Journal Article
• Authors: Lu, Xiaolin; Jiang, Ling; Zhang, Qizong; Huo, Junshen; Min, Jie; Liang, Qiuju; Zhao, Lijiao; Li, Yan; Chang, Shaoyan; Zhang, Ting; Zhang, Chunyi; Wang, Li
• Is Part Of: Journal of functional foods. Volume 99(2022)
• Publication Date: 2022-12
• Subjects: FA folic acid -- pABG p-aminobenzoy-l-glutamate -- SAM S-adenosylmethionine -- LINE-1 Long Interspersed Nucleotide Element-1 -- THF tetrahydrofolate -- UFA unmetabolized FA -- TUL tolerable upper limit -- DHFR dihydrofolate reductase -- FD folate-deficient -- FHC high-folate corn -- FH high FA -- FNC normal folate corn -- FN normal FA -- E embryonic day -- MRM multiple reaction monitoring -- SEM standard error of the mean; Folic Acid (FA) -- DNA Methylation -- High-Folate Corn -- Long Interspersed Nucleotide Element-1 (LINE-1) -- Lipid Metabolism; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2022.105317
• Languages: English
• ISSNs: 1756-4646

Abstract

Graphical abstract: Highlights: Folate-supplementation restored folate deficiency status in 4 weeks. Restoration effect varies depending on different FA sources. Orderly phospholipid metabolism implies a healthy status under FHC supplementation. Abstract: Since folic acid (FA) was officially recommended in 1998, concerns have emerged about the effect of excess FA intake, in particular with respect to negative clinical effects. Given this, a high-folate corn inbred line that we identified from a global germplasm collection was believed to become a valid alternative to FA supplementation. In this study, we proved this natural form of plant-sourced folates exhibited an effective restoration of folate deficiency. Folate metabolism and methylation modifications were restored in folate-deficient mice with folate-supplemented diets in 4 weeks. Restoration effects varies depending on folate sources, restricted retrotransposon activity and an orderly pattern of phospholipid/lipid metabolism protected by this high-folate corn partly ensures a healthy folate status for development. Totally, we proved in mice models that this kind of folate biofortification corn could provide a valid alternation of FA supplementation in alleviation of folate deficiency during pregnancy.