J-aggregates of Br- and piperazine-modified cyanine dye with the assistance of amphiphilic polypeptides for efficient NIR-IIa phototheranostics under 1064 nm irradiation. (December 2022)

• Record Type: Journal Article
• Authors: Teng, Changchang; Dang, Huiping; Zhang, Shangzhong; Xu, Yixuan; Yin, Dalong; Yan, Lifeng
• Is Part Of: Acta biomaterialia. Volume 154(2022)
• Page Start: 572
• Page End: 582
• Publication Date: 2022-12
• Subjects: J-aggregates -- Cyanine dye -- NIR-IIa fluorescence imaging -- Photothermal therapy; Biomedical materials -- Periodicals; 610.28
• DOI: 10.1016/j.actbio.2022.10.025
• Languages: English
• ISSNs: 1742-7061

Abstract

Abstract: The second near-infrared IIa window (NIR-IIa, 1300nm∼1400nm) enables high-resolution imaging and deep-tissue tumor treatment due to its unique low tissue scattering and autofluorescence, high temporal-spatial resolution, and deep tissue penetration. Therefore, NIR-IIa fluorescence imaging-guided phototherapy is of specific interest. However, organic dyes and their nanoparticles for NIR-IIa phototheranostics are still scarce. Here, we have synthesized a Br- and piperazine-modified cyanine dye (FN) and its nanomicelles encapsulated by an amphiphilic polypeptide with sidechains of tertiary amine (PEA). The J-aggregates of P@FN9 with 1116 nm absorption and efficient NIR-IIa fluorescence emission were formed by the self-assembly of FN and PEA. P@FN9 nanoparticles (NPs) showed good stability and high photothermal conversion efficiency (55.4%). In addition, the high spatial resolution and signal-to-background ratio (SBR) of P@FN9 were demonstrated by NIR-IIa fluorescence imaging of mouse vasculature. The P@FN9 NPs successfully performed the NIR-IIa fluorescence imaging-guided photothermal therapy, and both in vitro and in vivo experiments indicated that the P@FN9 NPs exhibited effective antitumor effects under the NIR-II (1064 nm) laser irradiation. Statement of significance: Br- and piperazine-modified cyanine dye, that showed NIT-II fluorescence, has been synthesized. After the dye was encapsulated by an amphiphilic polypeptide with sidechains of tertiary amine, J-aggregates were formed. The J-aggregates showed efficient fluorescence in the NIR-IIa region with high photothermal conversion efficiency. Under NIR-II 1064 light irradiation, the aggregates showed efficient NIR-IIa phototheranostics for tumors. Graphical abstract: Image, graphical abstract