CgCaspase-3 activates the translocation of CgGSDME in haemocytes of Pacific oyster Crassostrea gigas. Issue 131 (December 2022)

• Record Type: Journal Article
• Authors: Li, Xiaopeng; Yan, Xiaoxue; Leng, Jinyuan; Wang, Wei; Li, Yinan; Yang, Chuanyan; Sun, Jiejie; Wang, Lingling; Song, Linsheng
• Is Part Of: Fish & shellfish immunology. Issue 131(2022)
• Page Start: 757
• Page End: 765
• Publication Date: 2022-12
• Subjects: Crassostrea gigas -- Haemocytes -- Caspase-3 -- Gasdermin E -- Vibrio splendidus; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2022.10.036
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: Cysteinyl aspartate specific proteinase-3 (Caspase-3) is an important protein involved in the apoptosis and gasdermin E (GSDME)-mediated cell pyroptosis pathways in vertebrates. A Caspase-3 homologue (designated as Cg Caspase-3) was previously identified as an immune receptor specific for lipopolysaccharide (LPS) to regulate apoptosis in the Pacific oyster Crassostrea gigas . In the present study, the binding activity of Cg Caspase-3 to different pathogen associated molecular patterns (PAMPs) and its effects on Cg GSDME translocation in haemocytes were further investigated in C. gigas . The mRNA expression of Cg Caspase-3 could be detected in all the tested tissues, including hepatopancreas, labial palp, adductor muscle, gonad, gill, mantle and haemocytes, and it was highly expressed in labial palp, gonad, haemocytes, and adductor muscle. The mRNA expression of Cg Caspase-3 in haemocytes increased significantly at 3, 24, 48 and 72 h after LPS stimulation, and it increased significantly at 6, 12, 24 and 48 h after Vibrio splendidus stimulation. The recombinant Cg Caspase-3 displayed binding activity towards LPS, mannose (MAN), peptidoglycan (PGN), and polyinosinic-polycytidylic acid potassium salt (Poly (I:C)). The positive signals of Cg GSDME on haemocyte membrane became stronger at 3 h after V. splendidus stimulation, compared with that of Seawater group, and the co-localization of Cg Caspase-3 and Cg GSDME was observed in the haemocyte membrane. After the injection of ds Cg Caspase-3, the positive signals of Cg GSDME on haemocyte membrane became weaker compared with that of EGFP-RNAi group at 24 h after V. splendidus stimulation. The results suggested that Cg Caspase-3 was able to bind diverse PAMPs and activate the translocation of Cg GSDME in haemocytes of oyster response against pathogen invasion. Highlights: The mRNA expression of Cg Caspase-3 was detected in all tested tissues and it was induced by immune stimulation. Cg Caspase-3 displayed the binding activity to different PAMPs. Cg Caspase-3 was able to activate translocation of Cg GSDME in haemocytes to response against pathogen invasion.