Dietary supplementation of Pseudoalteromonas piscicida 1UB and fructooligosaccharide enhance growth performance and protect the whiteleg shrimp (Litopenaeus vannamei) against WSSV and Vibrio harveyi coinfection. Issue 131 (December 2022)

• Record Type: Journal Article
• Authors: Nababan, Yanti Inneke; Yuhana, Munti; Penataseputro, Tanjung; Nasrullah, Hasan; Alimuddin, Alimuddin; Widanarni, Widanarni
• Is Part Of: Fish & shellfish immunology. Issue 131(2022)
• Page Start: 746
• Page End: 756
• Publication Date: 2022-12
• Subjects: L. vannamei -- Synbiotic -- Growth -- Immunomodulation -- Disease resistance -- Pathogens coinfection; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2022.10.047
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: P. piscicida 1Ub and FOS were evaluated for their potential synbiotic effects on growth, immunological responses, and disease resistance against white spot syndrome virus and V. harveyi coinfection, the major pathogen in whiteleg shrimp aquaculture. Four different supplemented diets were used to feed the experimental shrimp for 40 days: control (control, no probiotic, and prebiotic), probiotic (PRO, P. piscisida 1UB 10 8 CFU mL −1 ), prebiotic (PRE, FOS 0.5% w/w), and the synbiotic (SYN, PRO + PRE). Shrimp's body weight, weight gain, specific growth rate, feed conversion ratio, survival, digestive enzyme activity, and metabolism-related gene expression were all evaluated on day 40. After 40 days, shrimp were infected with WSSV as the primary infection and V. harveyi as the secondary infection 24 h later. Shrimp were then grown for seven days and fed with a control diet. Survival, total hemocyte count (THC), differential hemocyte, phenol-oxidase (PO), respiratory burst activity (RB), and immune-gene expression were all analyzed at 0, 3, and 7 days after infection. The results showed that the PRO, PRE, and SYN supplementation improves whiteleg shrimp growth performance, immune responses, and protection against WSSV and V. harveyi coinfection. The increased activity of digestive enzymes and metabolism-related genes correlates with higher growth performance. The increase in THC, PO, RB, and immune-related gene expression after coinfection was associated with a significant reduction in shrimp mortality. Our findings also suggest that supplementing with synbiotics improves the overall performance of whiteleg shrimp significantly more than probiotics or prebiotics only. Highlights: Probiotic strain P. piscicida 1Ub combined with prebiotic FOS as synbiotics. Dietary synbiotics display a positive alteration of growth performance and immune responses in whiteleg shrimp. Dietary synbiotics significantly reduced shrimp mortality after WSSV and V. harveyi coinfection. Dietary synbiotics promotes a higher growth performance and disease protection than dietary probiotic or prebiotic.