Differential gene expression of red-spotted grouper (Epinephelus akaara) in response to lipopolysaccharide, poly I:C, and nervous necrosis virus revealed by RNA-seq data. Issue 131 (December 2022)

• Record Type: Journal Article
• Authors: Sandamalika, W.M. Gayashani; Liyanage, D.S.; Lim, Chaehyeon; Yang, Hyerim; Lee, Sukkyoung; Jeong, Taehyug; Wan, Qiang; Lee, Jehee
• Is Part Of: Fish & shellfish immunology. Issue 131(2022)
• Page Start: 939
• Page End: 944
• Publication Date: 2022-12
• Subjects: Red-spotted grouper -- Epinephelus akaara -- Differentially expressed genes -- RNA-seq; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2022.11.006
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: Red-spotted grouper ( Epinephelus akaara ) is a popular aquaculture species with high commercial value in the food industry. However, some infectious diseases may cause mass mortality in cultural practice. Therefore, it is important to understand the immune responses of red-spotted groupers upon pathogenic invasion to develop successful disease prevention mechanisms. Here, we analyzed the transcriptomic profiles of red-spotted grouper head kidney stimulated with lipopolysaccharide (LPS), polyinosinic:polycytidylic acid (poly I:C), and nervous necrosis virus (NNV) and identified differentially expressed genes (DEGs) using RNA-sequencing technology. Cluster analysis of the identified DEGs showed DEG distribution in nine separate clusters based on their expression patterns. However, significant upregulation of most DEGs was observed 6 h after poly I:C stimulation. The DEGs were functionally annotated using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis, which revealed significant expression of many immune-related signaling pathways, including antiviral, protein translation, cellular protein catabolic process, inflammatory responses, DNA repair, and cell division. Furthermore, selected DEGs were validated by quantitative real-time PCR, confirming the reliability of our findings. Collectively, this study provides insight into the immune responses of red-spotted groupers, thereby expanding the understanding of fish immunity. Highlights: The immune challenged head kidney transcriptomes of Epinephelus akaara were constructed using RNA-sequencing technology. A total of 4936 significant union DEGs were identified from the immune-challenged groups and control (PBS) group. Functional annotation of the DEGs were associated with immune-related signaling pathways. The quantitative real-time PCR results validated the reliability of the DEGs.