PLGA-PEG-fucoxanthin nanoparticles protect against ischemic stroke in vivo. (December 2022)

• Record Type: Journal Article
• Authors: Wang, Qiyao; Zhang, Difan; Lu, Jiani; Zhang, Jiaying; Xuan, Zhenquan; Gong, Luyun; Yang, Mengxiang; Jin, Lingli; Le, Jingyang; Zhu, An; Liang, Hongze; Benjamin Naman, C.; Zhang, Jinrong; Zhao, Li; He, Shan; Wang, Qinwen; Liu, Hao; Yan, Xiaojun; Zhao, Lingling; Cui, Wei
• Is Part Of: Journal of functional foods. Volume 99(2022)
• Publication Date: 2022-12
• Subjects: Fucoxanthin -- Nanoparticles -- Functional food -- Ischemic stroke -- Neurological disease -- Oxidative stress; ACSF artificial cerebrospinal fluid -- ANOVA analysis of variance -- BBB blood–brain barrier -- BCA bicinchoninic acid -- CAT catalase -- DAPI 4′, 6-diamidino-2-phenylindole -- DCM dichloromethane -- DMEM modified Eagle medium -- ELISA enzyme-linked immunosorbent assay -- ERK extracellular regulated protein kinases -- FBS fetal bovine serum -- FDA fluorescein diacetate -- GSH glutathione -- GSH-Px glutathione peroxidase -- DCF-DA 2′, 7′-dichlorodihydrofluorescein diacetate -- HT22 cells mouse hippocampal neuron cell line -- HO-1 heme oxygenase 1 -- HPLC high performance liquid chromatography -- IAA iodoacetic acid -- Iba-1 inized calcium binding adapter molecule 1 -- iNOS inducible nitric oxide synthase -- IκB inhibitor of kappa B -- LDH lactate dehydrogenase -- MCAO middle cerebral artery occlusion/reperfusion -- MTT 3-(4, 5-dimethylthiazol-2-yl)-2.5-diphenyltetrazolium bromide -- NF-κB nuclear factor-κB -- NO nitric oxide -- Nrf2 nuclear factor-E2-related factor 2 -- PEG polyethylene glycol -- PLGA poly lactic-co-glycolic acid -- PI propidium iodide -- PVA poly vinyl alcohol -- ROS reactive oxygen species -- SD Sprague-Dawley -- SEM scanning electron microscopy -- SOD superoxide dismutase -- SDS Sodium dodecyl sulfate -- SPF specific pathogen-free -- TBST tris buffered saline with tween -- TNF-α tumor necrosis factor alpha -- TTC 2, 3, 5-triphenyltetrazolium chloride -- UV ultraviolet visible; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2022.105359
• Languages: English
• ISSNs: 1756-4646

Abstract

Graphical abstract: Highlights: PLGA-PEG-fuc nanoparticles enhance brain bioavailability of fucoxanthin. PLGA-PEG-fuc nanoparticles prevent behavioral impairments in MCAO rats. PLGA-PEG-fuc nanoparticles inhibit neuroinflammation and oxidative stress. Abstract: Fucoxanthin is the most abundant marine carotenoid derived from edible brown seaweeds and has been used as a functional ingredient for the treatment of obesity. However, poor brain bioavailability of fucoxanthin has limited its application in combating neurological diseases. In this study, we demonstrated that 40 % fucoxanthin was released from polylactic- co -glycolic acid-polyethylene glycol (PLGA-PEG) nanoparticles at one day after incubation. Fucoxanthin nanoparticles (20–40 mg/kg, i.v. ) significantly prevented neurological and behavioral deficits in middle cerebral artery occlusion/reperfusion (MCAO)-treated rats via the reduction of M1 microglial activation, decrease of pro-inflammatory factor production, inactivation of the nuclear factor-κB (NF-κB) pathway, and elevation of the nuclear factor-E2-related factor 2 (Nrf2) pathway in ischemic penumbra. Moreover, fucoxanthin nanoparticles (5–15 μg/mL) inhibited hypoxia-stimulated neurotoxicity in a mouse hippocampal neuron cell line (HT22 cells). These results suggest that fucoxanthin nanoparticles enhanced brain bioavailability and produced neuroprotection against ischemic stroke in vivo with much lower effective concentrations than fucoxanthin itself, providing further support for the development of fucoxanthin as a functional ingredient for the prevention of ischemic stroke with proper nanoparticle formulations.