Safety evaluation study of lincomycin and spectinomycin hydrochloride intramuscular injection in chickens. (2022)

• Record Type: Journal Article
• Authors: Khan, Ejaz Ali; Ma, Jifei; Xiaobin, Meng; Jie, Yang; Mengyue, Liu; Hong, Liang; Shah, Luqman; Liu, Ailing
• Is Part Of: Toxicology reports. Volume 9(2022)
• Page Start: 204
• Page End: 209
• Publication Date: 2022
• Subjects: LC-SPH Lincomycin and spectinomycin hydrochloride -- i.m intramuscular -- VICH veterinary international cooperation on harmonization -- FCR feed conversion ratio -- CRD chronic respiratory disease -- GI gastrointestinal tract -- EU European Union -- MRLs maximum residue limits -- GLP good laboratory practice -- CREA creatinine -- GLU glucose -- TP total protein -- UREA blood urea nitrogen -- Ca calcium -- Rpm revolutions per minute -- EDTA ethylenediamine tetra-acetic acid -- WBC white blood cells -- RBC red blood cells -- HGB hemoglobin -- HCT hematocrit -- MCV mean corpuscular volume -- MCH mean corpuscular hemoglobin -- MCHC mean corpuscular hemoglobin concentration -- RDW coefficient of variation of red blood cell width -- PLT platelet count -- MPV mean platelet volume -- PDW platelet distribution width -- CK creatine kinase -- P phosphorus -- GLO globulin -- A/G albumin to globulin ratio; Safety evaluation -- Lincomycin and spectinomycin hydrochloride -- Chickens -- Clinical blood parameters -- Histopathology; Toxicology -- Periodicals; Clinical toxicology -- Periodicals; Drug-Related Side Effects and Adverse Reactions; Hazardous Substances; Poisoning; Toxicology; Electronic journals; Periodicals; Periodicals; 571.9505
• DOI: 10.1016/j.toxrep.2022.01.012
• Languages: English
• ISSNs: 2214-7500

Abstract

Graphical abstract: Highlights: Safety evaluation of antibacterial drugs combination study on target animals (Chickens). Combinations of two drugs, Lincomycin and spectinomycin hydrochloride (LC-SPH). Target animals were poultry birds. Lincomycin and spectinomycin hydrochlorides (LC-SPH) 20mg/kg body weight i.m injection investigated safe and recommended for a further clinical study. Abstract: This study aimed to investigate the nonclinical safety of lincomycin and spectinomycin hydrochloride (LC-SPH) intramuscular (i.m) doses on target animals (chickens) to provide guidelines for dose level design and side effect monitoring in clinical trials. A total of 80 healthy Arbor Acres plus broiler chicks were completely randomized and blindly divided into four treatment groups (control, one-time dose, three-time dose, and five-time dose) of 20 chicks each (20 chickens per group). At the age of day 15, all chickens (except the control group) were administered LC-SPH intramuscularly (chest muscles) at different doses of 20 mg/kg.bw, 60 mg/kg.bw, and 100 mg/kg.bw respectively for 9 consecutive days recommended by veterinary international cooperation on harmonization (VICH) guidelines. The chickens had ad libitum access to antibiotic-free feed and water. Feeding chickens were observed twice a day throughout the study. The drug safety was evaluated by complete blood count, biochemical parameters, histopathological, clinical signs, body weight gain, and feed conversion ratio (FCR). Hence, considering the minor toxicity of 60 mg/kg, our results reveal that intramuscular injection of at least 20 mg/kg body weight has no effects on growth performance, clinical blood parameters, organ coefficient, and histopathological parameters. Thus, a combination of LC-SPH 20 mg/kg body weight i.m injection investigated safe followed daily administration for nine consecutive days in healthy chickens. It is concluded that the experimental results support the safety of 20 mg/kg body weight in combination for the further clinical research study.