A C1q domain-containing protein in Pinctada fucata contributes to the innate immune response and elimination of the pathogen. Issue 131 (December 2022)

• Record Type: Journal Article
• Authors: Wang, Jing; Thaimuangphol, Wipavee; Chen, Zhiwei; Li, Guiying; Gong, Xiaoqing; Zhao, Mingming; Chen, Zongfa; Wang, Bei; Wang, Zhongliang
• Is Part Of: Fish & shellfish immunology. Issue 131(2022)
• Page Start: 582
• Page End: 589
• Publication Date: 2022-12
• Subjects: C1q domain-containing protein -- Pinctada fucata -- Innate immune system -- Phagocytosis -- siRNA; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2022.10.031
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: The C1q domain-containing proteins (C1qDCs) in bivalve mollusks primarily exist as the globular head C1q proteins (ghC1qs), for the N-terminal collagen domains were very rare in bivalves, although widespread in C1qDCs of vertebrates. In this work, the C1qDC protein with only a ghC1q domain (named as Pf-ghC1q ) was identified from Pinctada fucata, and molecular characterization, gene expression, and functional studies were also conducted. The full-length cDNA sequence of Pf-ghC1q was 738 bp long, containing a signal peptide of 23 residues encoded. Pf-ghC1q was clustered with some C1qDCs from other invertebrates in the phylogenetic tree analysis, rather than vertebrates. Pf-ghC1q was detected in all tested tissues, including the mantle, hemocyte, digestive gland, gill, and adductor muscle. Moreover, the expression levels of Pf-ghC1q were up-regulated in all tested tissues after the challenge with Vibrio alginolyticus 4 h later. The expression level of Pf-ghC1q was inhibited by specific si-276, and the low level of Pf-ghC1q affected the phagocytosis efficiency of V. alginolyticus by hemocytes. These results indicated that Pf-ghC1q may participate in the target recognition of V. alginolyticus and the phagocytosis process in the immune response of P. fucata . Highlights: The cDNA of a globular head C1q protein from Pinctada fucata was cloned and characterized. Pf-ghC1q was ubiquitous and highly expressed in gill and adductor muscle. Pf-ghC1q was responsive to Vibrio alginolyticus infection, and its expression level was highest at 2 h. Knockdown by specific siRNA suppressed the expression of Pf-ghC1q. Pf-ghC1q participated in the phagocytosis process and positively influenced phagocytic cells.