Oxygen-releasing hydrogels promote burn healing under hypoxic conditions. (December 2022)

• Record Type: Journal Article
• Authors: Bai, Que; Zheng, Caiyun; Sun, Na; Chen, Wenting; Gao, Qian; Liu, Jinxi; Hu, Fangfang; Zhou, Tong; Zhang, Yanni; Lu, Tingli
• Is Part Of: Acta biomaterialia. Volume 154(2022)
• Page Start: 231
• Page End: 243
• Publication Date: 2022-12
• Subjects: Self-healing -- Hypoxia -- Burn -- Angiogenesis -- Wound healing; GFs Growth factors -- ROS Reactive oxygen species -- OGM Oxygen-generating tissue scaffold -- GA Gallic acid -- HA Hyaluronic acid -- ECM Extracellular matrix -- OHA Oxidized hyaluronic acid -- CCK-8 Cell counting kit-8 -- DHE Dihydroethylamine -- TNF-α Transforming growth factor -- PECAM-1/CD31 Platelet endothelial cell adhesion molecule-1 -- α-SMA α-Smooth muscle actin; Biomedical materials -- Periodicals; 610.28
• DOI: 10.1016/j.actbio.2022.09.077
• Languages: English
• ISSNs: 1742-7061

Abstract

Abstract: Hypoxic nonhealing wounds are a common complication in chronic patients, and chronic hypoxia is the main reason for delayed wound healing, so local wound oxygenation may be an effective way to address this problem. Here, we proposed a system consisting of oxygen-releasing microsphere (GC) and self-healing hydrogel (QGO). QGO/GC hydrogel could promote survival, migration and tube formation of human umbilical vein endothelial cells under hypoxic conditions. Moreover, QGO/GC hydrogels exhibited biocompatibility in vitro and in vivo . The hypoxic mouse burn model further confirmed that QGO/GC hydrogel could promote tissue repair by reducing inflammation (TNF-α and IL-1β), increasing angiogenesis (CD31, VEGF and α-SMA) and collagen deposition. This study provided an effective oxygen-releasing hydrogel that could offer a simple and effective method for the clinical treatment of chronic hypoxic wounds. Statement of significance: Burn injury is caused by various exogenous factors such as friction, cold, radiations, electricity, chemicals, hot surfaces or liquids. Severe burn can damage the entire skin layer, and the healing process is delayed due to an unbalanced inflammatory response, excessive reactive oxygen species, lack of angiogenesis (insufficient nutrient and oxygen availability), and susceptibility to infection. In the present study, we proposed an oxygen-releasing hydrogel (QGO/GC). QGO/GC hydrogel could promote survival, migration, and tube formation of human umbilical vein endothelial cells under hypoxic conditions. And QGO/GC hydrogels could promote tissue repair by reducing inflammation, increasing angiogenesis and collagen deposition. This work provided an effective oxygen-releasing hydrogel for the clinical management of chronic hypoxic wounds. Graphical abstract: Image, graphical abstract