Cellular basis of differential endochondral growth in Lake Malawi cichlids. Issue 12 (30th August 2022)

• Record Type: Journal Article
• Authors: Johnson, Savannah; Heubel, Brian; Bredesen, Carson; Schilling, Thomas; Le Pabic, Pierre
• Is Part Of: Developmental dynamics. Volume 251:Issue 12(2022)
• Page Start: 2001
• Page End: 2014
• Publication Date: 2022-08-30
• Subjects: cartilage -- chondrocyte -- craniofacial -- hypertrophy -- skeleton -- teleosts; Morphogenesis -- Periodicals; Anatomy -- Periodicals; Anatomie -- Périodiques; Biologie du développement -- Périodiques; 571.833
• DOI: 10.1002/dvdy.529
• Languages: English
• ISSNs: 1058-8388

Abstract

Abstract: Background: The shape and size of skeletal elements is determined by embryonic patterning mechanisms as well as localized growth and remodeling during post‐embryonic development. Differential growth between endochondral growth plates underlies many aspects of morphological diversity in tetrapods but has not been investigated in ray‐finned fishes. We examined endochondral growth rates in the craniofacial skeletons of two cichlid species from Lake Malawi that acquire species‐specific morphological differences during postembryonic development and quantified cellular mechanisms underlying differential growth both within and between species. Results: Cichlid endochondral growth rates vary greatly (50%‐60%) between different growth zones within a species, between different stages for the same growth zone, and between homologous growth zones in different species. Differences in cell proliferation and/or cell enlargement underlie much of this differential growth, albeit in different proportions. Strikingly, differences in extracellular matrix production do not correlate with growth rate differences. Conclusions: Differential endochondral growth drives many aspects of craniofacial morphological diversity in cichlids. Cellular proliferation and enlargement, but not extracellular matrix deposition, underlie this differential growth and this appears conserved in Osteichthyes. Cell enlargement is observed in some but not all cichlid growth zones and the degree to which it occurs resembles slower growing mammalian growth plates. Key Findings: Growth rates vary greatly between cichlid endochondral growth zones. Differences in growth rate of the same growth zone are detected between stages and species, and between different growth zones within species. Cell proliferation is the main contributor to endochondral growth and its variation in cichlids. Unlike in tetrapods, cell enlargement is only detected in some cichlid growth zones.