Transduction of Herpesvirus saimiri‐Transformed T Cells with Exogenous Genes of Interest. (1st November 2016)

• Record Type: Journal Article
• Authors: Martínez‐Barricarte, Rubén; de Jong, Sarah Jill; Markle, Janet; de Paus, Roel; Boisson‐Dupuis, Stephanie; Bustamante, Jacinta; van de Vosse, Esther; Fleckenstein, Bernhard; Casanova, Jean‐Laurent
• Editors: Coligan, John E.; Bierer, Barbara E.; Margulies, David H.; Shevach, Ethan M.; Strober, Warren
• Is Part Of: Current protocols in immunology. Volume 115(2016)
• Page Start: 7.21C.1
• Page End: 7.21C.12
• Publication Date: 2016-11-01
• Subjects: herpesvirus saimiri‐transformed T cells -- HVS‐transformed T cell -- retrovirus -- transduction -- T cells; Immunology -- Laboratory manuals; Medical protocols; Immunologic Techniques; Medical protocols; Immunology; Laboratory manuals; Reference works; 571.96028
• DOI: 10.1002/cpim.15
• Languages: English
• ISSNs: 1934-3671

Abstract

Abstract: Human T cells can be transformed and expanded with herpesvirus saimiri (HVS). HVS‐transformed T cells from patients have facilitated the study of a broad range of primary immunodeficiencies (PID) in which T‐cell development or function is altered. However, the utility of HVS‐transformed T cells for genetic studies has been limited by technical challenges in the expression of exogenous genes, including wild‐type or mutant alleles. A novel, gamma retrovirus–based method for the simple and reliable transduction, purification, and study of HVS‐transformed T cells is described. © 2016 by John Wiley & Sons, Inc.