Venipuncture Procedures: Sources of Human Peripheral Blood Cells, Serum, and Plasma. (13th February 2018)

• Record Type: Journal Article
• Authors: Bierer, Barbara E.; Strober, Warren
• Editors: Coligan, John E.; Bierer, Barbara E.; Margulies, David H.; Shevach, Ethan M.; Strober, Warren
• Is Part Of: Current protocols in immunology. Volume 118(2017)
• Page Start: A.3F.1
• Page End: A.3F.5
• Publication Date: 2018-02-13
• Subjects: lymphapheresis -- peripheral blood -- plasma -- serum -- venipuncture; Immunology -- Laboratory manuals; Medical protocols; Immunologic Techniques; Medical protocols; Immunology; Laboratory manuals; Reference works; 571.96028
• DOI: 10.1002/cpim.27
• Languages: English
• ISSNs: 1934-3671

Abstract

Abstract: Peripheral blood is the chief source of mononuclear cells (lymphocytes and monocytes) for immunologic studies of humans. This appendix presents protocols for obtaining blood by simple venipuncture when relatively small amounts of blood (10 to 100 ml) are necessary or by lymphapheresis when large amounts (300 to 5000 ml) are necessary. Cells collected by these procedures can be further separated by techniques described in Chapter 7. © 2017 by John Wiley & Sons, Inc.