Zinc oxide nanoparticles mediated biostimulant impact on cadmium detoxification and in silico analysis of zinc oxide-cadmium networks in Zea mays L. regulome. (1st January 2023)

• Record Type: Journal Article
• Authors: Tanveer, Yashfa; Jahangir, Saman; Shah, Zafar Abbas; Yasmin, Humaira; Nosheen, Asia; Hassan, Muhammad Nadeem; Illyas, Noshin; Bajguz, Andrzej; El-Sheikh, Mohamed A.; Ahmad, Parvaiz
• Is Part Of: Environmental pollution. Volume 316(2023)part 2
• Publication Date: 2023-01-01
• Subjects: Biostimulant mediated Cd remediation -- Cadmium detoxification -- Zinc oxide nanoparticles -- Cadmium remediation; Pollution -- Periodicals; Pollution -- Environmental aspects -- Periodicals; Environmental Pollution -- Periodicals; Pollution -- Périodiques; Pollution -- Aspect de l'environnement -- Périodiques; Pollution -- Effets physiologiques -- Périodiques; Pollution; Pollution -- Environmental aspects; Periodicals; Electronic journals; 363.73
• DOI: 10.1016/j.envpol.2022.120641
• Languages: English
• ISSNs: 0269-7491

Abstract

Abstract: Cadmium (Cd) toxicity can significantly limit plant growth and development. To eliminate the toxic effects of Cd stress, we intended to evaluate the biochemical mediated physiological responses in maize treated with biostimulant and zinc oxide nanoparticles (ZnPs). In silico analysis exhibited that the maize treated with Cd stress (200 μM) had an adverse impact on CAT1, CAT2, CAT3 and gor1 proteins, which are influential in managing the machinery of redox homeostasis. While maize inoculated with bacteria-based biostimulant and ZnPs (10 ppm) showed prominently improved biomass, chlorophyll a, b and carotenoid content. We found a significant increase in the total sugar, protein, proline content and antioxidants under the effect of Cd stress. However, these parameters are further enhanced by applying biostimulants and ZnPs. Declined lipid peroxidation and membrane solubilization index under the effect of biostimulant and ZnPs was observed. Furthermore, these treatments improved maize's zinc, copper, sodium, magnesium, iron, potassium and calcium content. Based on these results, an antagonistic relationship between Zn and Cd uptake that triggered efficient Cd detoxification in maize shoot was found. Scanning electron micrography showed distorted leaf structure of the Cd stressed plants while the biostimulant and ZnPs reduced the structural cell damage of maize leaves. In silico study showed that ZnO positively regulates all protein interactors, including GRMZM2G317386_P01 (Metallo endo proteinase 1-MMP), GRMZM2G110220_P01 (Metallo endo proteinase 5-MMP), GRMZM2G103055_P01 (Alpha-amylase) and GRMZM2G006069_P01 (Zn-dependent exo peptidase superfamily) proteins which are involved in energy generating processes, channels formation, matrix re-localization and stress response. This suggests that ZnO offers an ideal role with protein interactors in maize. Our findings depict that these treatments, i.e., biostimulant and ZnPs alone, are efficient enough to exhibit Cd remediation potential in maize; however, their combination showed synergistic effects. Highlights: Cadmium (Cd) stress restricted maize growth through dysfunctional biochemical processes. PGPB-based biostimulant-assisted Cd remediation offers a green solution for sustainable plant growth. ZnPs mediated biostimulant reduced Cd accumulation by modulating Cd-influenced maize physiology In silico pipeline investigated interactome level participation of ZnO-Cd mediated protein interactions in maize.