The Expression of αvβ3 and Osteopontin in Osteoarthritic Knee Cartilage and Their Correlations With Disease Severity and Chondrocyte Senescence. Issue 1 (20th January 2023)

• Record Type: Journal Article
• Authors: Cheng, Chao; Tian, Jian; Gao, Shu-Guang; Zhou, Zhi-Hong; Yang, Rui-Qi; Xiao, Kai; Guo, Wei; Liu, Li; Yang, Hua; Zhang, Fang-Jie
• Is Part Of: Applied immunohistochemistry & molecular morphology. Volume 31:Issue 1(2023)
• Page Start: 57
• Page End: 63
• Publication Date: 2023-01-20
• Subjects: osteopontin -- chondrosenescence -- chondrocyte -- αvβ3 integrin -- senescent-associated-β-galactosidase -- osteoarthritis; Diagnostic immunohistochemistry -- Periodicals; Immunohistochemistry -- Periodicals; Cells -- Morphology -- Periodicals; Molecular diagnosis -- Periodicals; 616.079
• DOI: 10.1097/PAI.0000000000001063
• Languages: English
• ISSNs: 1541-2016

Abstract

Abstract : Osteoarthritis (OA) is the main joint disease associated with aging. Previous studies have confirmed that both osteopontin (OPN) and αvβ3 integrin are involved in the progression of knee OA. The purpose of this study was to determine the expression of OPN and αvβ3 integrin and chondrocyte senescence levels in OA. Forty-six cartilage tissues from normal and knee OA patients were divided into 4 groups of normal, minor, moderate, and severe lesions based on the Mankin score. Immunohistochemistry and western blotting were used to determine the expression of αvβ3, OPN, and senescent-associated-β-galactosidase (SAβ-gal) in articular cartilage. Then, Spearman's correlation was used to analyze the correlations between the Mankin scores and αvβ3, OPN and SAβ-gal. Pearson correlation analysis was used to analyze the correlations among αvβ3, OPN, and SAβ-gal. The expression of OPN, αvβ3, and SAβ-gal in articular cartilage was explored. αvβ3, OPN, and SAβ-gal proteins were all elevated in OA cartilage, and the correlation coefficient between the Mankin score and the average optical density value of αvβ3, OPN, SAβ-gal were r =0.60, r =0.75, and r =0.87, respectively, all P <0.001; the correlation between the average optical density value of αvβ3 and OPN was r =0.3191, P <0.05; the correlation between αvβ3 and SAβ-gal was r =0.4955, P <0.001; and the correlation between OPN and SAβ-gal was r =0.7821, P <0.001. The correlations among αvβ3, OPN, and SAβ-gal expression in articular cartilage might be important in OA progression and pathogenesis. Nonetheless, more research is needed to elucidate the exact contribution of αvβ3, OPN, and SAβ-gal to the degenerative process of OA.