Keratin intrinsic fluorescence as a mechanism for non-invasive monitoring of its glycation. (1st January 2023)

• Record Type: Journal Article
• Authors: Muir, Rhona; Forbes, Shareen; Birch, David J S; Vyshemirsky, Vladislav; Rolinski, Olaf J
• Is Part Of: Methods and applications in fluorescence. Volume 11:Number 1(2023)
• Publication Date: 2023-01-01
• Subjects: keratin -- glucose -- protein glycation -- intrinsic fluorescence -- time-resolved fluorescence spectroscopy; Fluorescence spectroscopy -- Periodicals; Fluorescence -- Periodicals; Spectrometry, Fluorescence -- Periodicals; Fluorescence -- Periodicals; Fluorescence; Fluorescence spectroscopy; Periodicals; 535.352
• DOI: 10.1088/2050-6120/aca507
• Languages: English
• ISSNs: 2050-6120

Abstract

Abstract: We have studied the evolution of keratin intrinsic fluorescence as an indicator of its glycation. Steady-state and time-resolved fluorescence of free keratin and keratin-glucose samples were detected in PBS solutions in vitro . The changes in the fluorescence response demonstrate that the effect of glucose is manifest in the accelerated formation of fluorescent cross-links with an emission peak at 460 nm and formation of new cross-links with emission peaks at 525 nm and 575 nm. The fluorescence kinetics of these structures is studied and their potential application for the detection of long-term complications of diabetes discussed.