Deciphering the underlying wound healing mechanisms of Streptocaulon juventas (Lour.) Merr. by integrating network pharmacology, transcriptomics, and experimental validation. (10th February 2023)

• Record Type: Journal Article
• Authors: Li, Yang; Zang, Jie; Wang, Xuemei; Feng, Xinchi; Qiu, Feng
• Is Part Of: Journal of ethnopharmacology. Volume 302:Part A(2023)
• Publication Date: 2023-02-10
• Subjects: Streptocaulon juventas (Lour.) Merr. -- Wound healing -- L929 -- AKT-mTOR signaling pathway -- ERK-p38 signaling pathway; α-SMA α-Smooth Muscle Actin -- AKT protein kinase B -- BCA bicinchoninic acid -- ANOVA analysis of variance -- BP Biological Process -- CC Cellular Component -- DEGs differential expression genes -- DMEM Dulbecco's modified Eagle's medium -- ECL electrochemiluminescence -- ECM extracellular matrix -- ERK extracellular signal-regulated kinases -- ESI electrospray ionization -- ESJ extract of Streptocaulon juventas (Lour.) Merr. -- FBS fetal bovine serum -- FDR false discovery rate -- HE hematoxylin eosin -- Hyp Hydroxyproline -- LSD least significant difference -- MAPK mitogen-activated protein kinase -- MF Molecular Function -- mTOR mammalian target of rapamycin -- MTT 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2-H-tetrazolium bromide -- PBS phosphate buffer saline -- PI3K phosphatidylinositol 3 kinase -- PPI protein-protein interactions -- PVDF polyvinylidene difluoride -- rb-FGF recombinant bovine basic fibroblast growth factor -- SD standard deviation -- SEA Similarity ensemble approach -- SJ Streptocaulon juventas (Lour.) Merr. -- TBST Tris-buffered saline -- TPM transcripts per million -- TTD Therapeutic Target Database; Ethnopharmacology -- Periodicals; Pharmacognosy -- Periodicals; Herbs -- Periodicals; Herbs -- Periodicals; Pharmacognosy -- Periodicals; Pharmacognosie -- Périodiques; Herbes -- Périodiques; 615.1
• DOI: 10.1016/j.jep.2022.115890
• Languages: English
• ISSNs: 0378-8741

Abstract

Abstract: Ethnopharmacological relevance: Streptocaulon juventas (Lour.) Merr. (SJ), a traditional Chinese folk medicine, has been widely used for the treatment of dysentery and traumatic injuries since ancient times. However, the mechanisms underlying its wound healing activity remain unclear. Aim of the study: The aim of this study was to evaluate the wound healing activity of SJ and clarify the underlying molecular mechanisms. Materials and methods: The wound healing activity of an ethanol extract of SJ (ESJ) was confirmed in rat full-thickness wound models. UPLC-Q-TOF-MS/MS was used to analyze the composition of ESJ. Potential molecular targets and signaling pathways involved in the wound healing activity of ESJ were predicted using network pharmacology and transcriptomic analyses. In addition, the L929 cells were used to evaluate the in vitro wound healing activity of ESJ and to verify the predicted pathways. Results: In rat wound models, ESJ significantly accelerated wound healing and promoted hydroxyproline production in wounds. Network pharmacology and transcriptomic analyses results revealed that ESJ might promote wound healing by activating the AKT and MAPK pathways. In L929 cells, ESJ significantly promoted cell proliferation, migration, and expression of collagen I and α-SMA. Additionally, ESJ treatment increased the phosphorylation of AKT, mTOR, ERK, and p38 in a time- and dose-dependent manner. Conclusions: ESJ significantly promoted wound healing in vivo and in vitro . AKT-mTOR and ERK-p38 signaling pathways were involved in the wound healing activity of ESJ. Graphical abstract: Image 1