Chemical composition and effects of ethanolic extract and gel of Cochlospermum regium (Schrank) Pilg. Leaves on inflammation, pain, and wounds. (10th February 2023)

• Record Type: Journal Article
• Authors: Galvão, Fernanda; dos Santos, Elisangela; Gomes da Silva Dantas, Fabiana; Irlan da Silva Santos, José; da Paz Costa Sauda, Talita; Carvalho dos Santos, Ariany; Carvalho Souza, Roosevelt Isaias; da Silva Pinto, Luciano; Ferreira Moraes, Carlos André; Sangalli, Andréia; Leite Kassuya, Candida Aparecida; Nogueira, Cláudio Rodrigo; Pires de Oliveira, Kelly Mari
• Is Part Of: Journal of ethnopharmacology. Volume 302:Part A(2023)
• Publication Date: 2023-02-10
• Subjects: Algodãozinho do cerrado -- Anti-inflammatory -- Wound healing -- Anti-nociceptive -- Chemical composition -- Cochlospermum regium leaves; Ethnopharmacology -- Periodicals; Pharmacognosy -- Periodicals; Herbs -- Periodicals; Herbs -- Periodicals; Pharmacognosy -- Periodicals; Pharmacognosie -- Périodiques; Herbes -- Périodiques; 615.1
• DOI: 10.1016/j.jep.2022.115881
• Languages: English
• ISSNs: 0378-8741

Abstract

Abstract: Ethnopharmacological relevance: Cochlospermum regium is well-known as "Algodãozinho do cerrado" in folk Brazilian medicine, and is used to fight infections, inflammation and skin disorders. Aim of the study: To identify the phytochemical constituents and the effects of the ethanolic extract of C. regium leaves (EECR) on inflammation and pain, and the effects of C. regium gel (GEECR) on wound healing. Materials and methods: Animals were treated with EECR (30–300 mg/kg) or GEECR (1.25 and 2.5%) and studies were conducted using carrageenan-induced pleurisy and paw edema tests, formalin-induced pain model, and excision wound model. Results: In total, 25 compounds, including quercitrin, methyl gallate, and 1, 2, 3, 4, 6-pentagalloylhexose, with highest detectability were identified. The treatments reduced leukocyte migration, nitric oxide production, protein extravasation, edema, mechanical hyperalgesia, pain in both phases (neurogenic and inflammatory), cold hypersensitivity, and improved wound closure and tissue regeneration. Conclusions: The present findings established the anti-inflammatory, anti-nociceptive, and wound healing potential of the leaves of C. regium, confirming the potential therapeutic effect of this plant. Graphical abstract: Image 1 Highlights: Cochlospermum regium leaves contain 25 compounds with biological activities. Quercitrin, methyl gallate and 1, 2, 3, 4, 6-pentagalloylhexose were the most detectable. The leaf extract possess anti-inflammatory and anti-nociceptive properties. The gel-formulated extract improves wound closure and tissue regeneration.