Combination of BauA and OmpA elicit immunoprotection against Acinetobacter baumannii in a murine sepsis model. (December 2022)

• Record Type: Journal Article
• Authors: Tamehri, Motahare; Rasooli, Iraj; Pishgahi, Mahdi; Jahangiri, Abolfazl; Ramezanalizadeh, Fatemeh; Banisaeed Langroodi, Seyedeh Reyhaneh
• Is Part Of: Microbial pathogenesis. Volume 173(2022)Part A
• Publication Date: 2022-12
• Subjects: Acinetobacter baumannii. -- BauA. -- Immunogenicity. -- OmpA. -- Vaccine; Pathogenic microorganisms -- Periodicals; Pathology, Molecular -- Periodicals; Communicable Diseases -- microbiology -- Periodicals; Communicable Diseases -- parasitology -- Periodicals; Micro-organismes pathogènes -- Périodiques; Pathologie moléculaire -- Périodiques; Electronic journals; 616.9041
• DOI: 10.1016/j.micpath.2022.105874
• Languages: English
• ISSNs: 0882-4010

Abstract

Abstract: Aims: Acinetobacter baumannii causes severe nosocomial infections and is a difficult-to-treat pathogen due to the development of multidrug-resistant (MDR) strains. Vaccines and antibody therapy represent alternative promising strategies for the control of infections caused by A. baumannii or its MDR strains. OmpA and BauA have been assigned as protective antigens. However, the efficacy of the combination of these antigens is yet to be investigated. In this study, we targeted two critical antigens of A. baumannii (BauA and OmpA) to enhance immunoprotecting against A. baumannii . Methods and results: The recombinant BauA and OmpA were expressed and purified. The purified proteins were administered to BALB/c mice alone and in combination. Immune sera were assessed against BauA, OmpA and two constructs harboring immunogenic loops of these antigen. The mice were then challenged with a clinical isolate of A. baumannii . Indirect ELISA confirmed significant antibody rise to the antigens. The immunogenic loops were detected in the hybrid construct. The specific sera detected OmpA, BauA and constructs harboring immunogenic loops of these antigen with different affinities. A significant decrease in the bacterial loads was noted in the spleen, liver, and lungs of the immunized mice groups. However, the group received combination of BauA and OmpA showed lower bacterial burden in the spleen and liver. Conclusions: Combination of BauA and OmpA enhances immunoprotection against A. baumannii infections. Highlights: Combination of BauA and OmpA was administered to BALB/c mice. Indirect ELISA confirmed significant antibody rise to the antigen. Significant decrease in the bacterial loads was noted in organs of the immunized mice. Immune sera detected BauA, OmpA, and their immunogenic loops in a hybrid construct. Combination of BauA and OmpA enhances protection against A. baumannii infections.