Anti-inflammatory and anti-pathogenic potential of Lacticaseibacillus rhamnosus IDCC 3201 isolated from feces of breast-fed infants. (December 2022)

• Record Type: Journal Article
• Authors: Chae, Seung A; Ramakrishnan, Sudha Rani; Kim, Taeok; Kim, So-Rim; Bang, Won Yeong; Jeong, Chae-Rim; Yang, Jungwoo; Kim, Soo-Jung
• Is Part Of: Microbial pathogenesis. Volume 173(2022)Part A
• Publication Date: 2022-12
• Subjects: Anti-inflammatory -- Anti-pathogenic -- Lactic acid bacteria -- Lacticaseibacillus rhamnosus -- Probiotics; Pathogenic microorganisms -- Periodicals; Pathology, Molecular -- Periodicals; Communicable Diseases -- microbiology -- Periodicals; Communicable Diseases -- parasitology -- Periodicals; Micro-organismes pathogènes -- Périodiques; Pathologie moléculaire -- Périodiques; Electronic journals; 616.9041
• DOI: 10.1016/j.micpath.2022.105857
• Languages: English
• ISSNs: 0882-4010

Abstract

Abstract: Objective: We investigated the anti-inflammatory and anti-pathogenic activities of Lacticaseibacillus rhamnosus IDCC 3201 isolated from the feces of breast-fed infants. Methods: Cell viability, nitric oxide (NO) production, and expression of inflammatory markers by L. rhamnosus IDCC 3201 were quantitatively analyzed in lipopolysaccharide (LPS)-induced RAW 264.7 macrophages. The antibacterial and antifungal activities of L. rhamnosus IDCC 3201 against various pathogens were also investigated. Results: Treatment of LPS-induced macrophages with cell-free supernatant of L. rhamnosus IDCC 3201 significantly decreased the expression levels of tumor necrosis factor (TNF-α) and interleukin-6 (IL-6). Nitric oxide synthase (iNOS) and cyclooxygenase (COX-2) levels also significantly decreased in LPS-induced macrophages. Phenotypically, the treatment of L. rhamnosus IDCC 3201 reduced the production of nitric oxide (NO) in LPS-induced macrophages. Furthermore, L. rhamnosus IDCC 3201 was proven to have potent inhibitory activities against various pathogens responsible for inflammatory responses in the gastrointestinal tract (i.e., Bacillus cereus, Enterococcus faecalis, Staphylococcus aureus, and Salmonella Typhimurium), respiratory system (i.e., Streptococcus pneumoniae ), and vagina (i.e., Candida albicans ). Conclusion: L. rhamnosus IDCC 3201 has anti-inflammatory activity in terms of decreased expression of cytokines, inflammation-inducible enzymes in LPS-induced macrophages, and anti-pathogenic activity. Graphical abstract: Image 1 Highlights: L. rhamnosus IDCC 3201 was isolated from the feces of breast-fed infants. The strain has anti-inflammatory activity related to nitric oxide (NO) production. Anti-inflammatory activity to cytokines and cytokine-induced enzymes was observed. L. rhamnosus IDCC 3201 shows anti-pathogenic activity against various pathogens. The strain is promising post-probiotics with anti-inflammation and anti-pathogenesis.