Development of an In Vitro Method to Estimate the Sensitization Induction Level of Contact Allergens. Issue 1 (21st February 2018)

• Record Type: Journal Article
• Authors: Galbiati, Valentina; Gibbs, Sue; Roggen, Erwin; Corsini, Emanuela
• Editors: Costa, Lucio G; Davila, Julio C.; Lawrence, David A.; Reed, Donald J.
• Is Part Of: Current protocols in toxicology. Volume 75:Issue 1(2018)
• Page Start: 20.15.1
• Page End: 20.15.20
• Publication Date: 2018-02-21
• Subjects: reconstituted human epidermis -- 3D model -- in vitro -- contact allergens -- potency -- contact hypersensitivity; Toxicology -- Laboratory manuals; Toxicology -- methods; Toxicology; Laboratory Manual; Electronic books; Laboratory manuals; 571.95028
• DOI: 10.1002/cptx.44
• Languages: English
• ISSNs: 1934-9254

Abstract

Abstract: Recently, an in vitro procedure, which combines the epidermal equivalent potency assay with assessment of IL‐18 to provide a single test for identification and classification of skin sensitizers, was developed and validated. This unit will describe a simple in vitro method for estimation of the expected sensitization induction level interpolating in vitro EC50 and IL‐18 SI2 values to predict LLNA EC3 and/or human NOEL from standards curves generated using reference contact allergens, based on the use of Reconstituted human Epidermis (RhE).© 2018 by John Wiley & Sons, Inc.