Isolation of Endothelial Cells from Mouse Lung. Issue 1 (7th August 2014)

• Record Type: Journal Article
• Authors: Cao, Gaoyuan; Abraham, Valsamma; DeLisser, Horace M.
• Editors: Costa, Lucio G; Davila, Julio C.; Lawrence, David A.; Reed, Donald J.
• Is Part Of: Current protocols in toxicology. Volume 61:Issue 1(2014)
• Page Start: 24.2.1
• Page End: 24.2.9
• Publication Date: 2014-08-07
• Subjects: endothelial cells -- microvascular cells -- lung cells; Toxicology -- Laboratory manuals; Toxicology -- methods; Toxicology; Laboratory Manual; Electronic books; Laboratory manuals; 571.95028
• DOI: 10.1002/0471140856.tx2402s61
• Languages: English
• ISSNs: 1934-9254

Abstract

Abstract: The isolation of endothelial cells (ECs) from knockout and transgenic mouse lines provides the opportunity to study the endothelial‐specific activities of a targeted molecule. As a means of pursuing these types of investigations, the protocols described in this unit provide a reliable method for isolating lung microvascular ECs from mouse neonatal pups that can be serially passaged. These protocols are useful in settings where mouse age is irrelevant and a pure population of pulmonary vascular ECs, uncontaminated by other cells, is needed. When a specific source of ECs is not required, these procedures also represent a reliable means of obtaining murine ECs in general. Curr. Protoc. Toxicol . 61:24.2.1‐24.2.9. © 2014 by John Wiley & Sons, Inc.